IST2: an insertion sequence from Thiobacillus ferrooxidans.
IST2: an insertion sequence from Thiobacillus ferrooxidans.
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IST2：来自氧化亚铁硫杆菌的插入序列。
DOI：
10.1073/pnas.85.19.7284
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发表时间：
1988
影响因子：
11.1
通讯作者：
D. Holmes
中科院分区：
文献类型：
--
作者：
J. R. Yates;R. P. Cunningham;D. Holmes
氧化亚铁硫杆菌(ATCC 19859)基因组至少含有两个重复序列家族，命名为家族1和家族2。测定了家族2的核苷酸序列。它长1408个碱基对，具有与插入序列(IS元件)相似的结构特征。末端反向重复序列长度为25个碱基对。这些反向重复序列并不完美，在长度上与目标位点的9个碱基对相邻。检测到几个开放阅读框(最长为888个碱基对)。我们已将其命名为类元素序列IST2。分析了IST2的第二个例子的末端，并与第一个例子的末端进行了比较。DNA序列是相同的，并且存在类似大小的靶点复制。
The genome of Thiobacillus ferrooxidans (strain ATCC 19859) contains at least two families of repeated sequences, termed family 1 and 2. The nucleotide sequence of a family 2 member was determined. It is 1408 base pairs long and has structural features similar to those of insertion sequences (IS elements). Terminal inverted repeats 25 base pairs in length are present. These inverted repeats are imperfect and adjacent to target-site duplications 9 base pairs in length. Several open reading frames were detected (the longest was 888 base pairs). We have named this IS element-like sequence IST2. The ends of a second example of IST2 were analyzed and compared to those of the first. The DNA sequences are identical and similarly sized target-site duplications are present.