Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.
Construction of vectors and a genomic library for use with his3-deficient strains of Schizosaccharomyces pombe.
复制标题
构建用于his3缺陷型粟酒裂殖酵母菌株的载体和基因组文库。
DOI：
10.1016/0378-1119(96)00085-6
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
Gould,KL
中科院分区：
文献类型：
--
作者：
Ohi,R;Feoktistova,A;Gould,KL
描述了使用his3+基因作为选择标记构建用于粟酒裂殖酵母的载体。此外，我们报告了在含有His3+的穿梭载体中构建基因组文库，以通过在His3活性缺陷的菌株中互补突变体功能来促进基因的克隆。
The construction of vectors for use in Schizosaccharomyces pombe using the his3+gene as a selectable marker is described. In addition, we report the construction of a genomic library in a his3+containing shuttle vector to facilitate the cloning of genes by complementation of mutant function in strains defective for His3 activity.