Expressed sequence tags in cultivated peanut (Arachis hypogaea): discovery of genes in seed development and response to Ralstonia solanacearum challenge
Expressed sequence tags in cultivated peanut (Arachis hypogaea): discovery of genes in seed development and response to Ralstonia solanacearum challenge
复制标题
栽培花生（Arachishypogaea）中表达的序列标签：种子发育中基因的发现和对青枯菌挑战的反应
DOI：
10.1007/s10265-012-0491-9
复制
发表时间：
2012-11-01
影响因子：
2.8
通讯作者：
Liao, Boshou
中科院分区：
文献类型：
--
作者：
Huang, Jiaquan;Yan, Liying;Liao, Boshou
尽管花生是一种重要的油料作物，但它只有162，030个表达序列标签（EST）公开可用，其中86，943个来自栽培植物。需要从栽培花生中获得更多的EST，以分离抗逆、组织特异性和发育重要基因。在此，我们从我们构建的5个花生青枯雷尔氏菌（Ralstonia solanacearum）感染根的cDNA文库中获得了63，234个EST。青枯菌侵染的叶和未侵染的培养花生根、叶和发育中的种子。在这些EST中，有14，547个独特序列，7，961个暂定共有序列和6，586个单例序列。鉴定了47.8%的序列的推定功能，包括转录因子、组织特异性基因、参与脂肪酸生物合成和油形成调节的基因以及抗性基因类似物基因。此外，还鉴定了花生叶片和根中的差异表达基因，包括参与乙烯和茉莉酸信号转导途径的基因。青枯菌挑战样品。来自不同花生组织的大表达数据集将是标记开发和基因表达分析的有价值的来源。这也将有助于寻找脂肪酸合成和油脂形成调控的候选基因，以及研究花生寄主与R.青枯病菌
Although an important oil crop, peanut has only 162,030 expressed sequence tags (ESTs) publicly available, 86,943 of which are from cultivated plants. More ESTs from cultivated peanuts are needed for isolation of stress-resistant, tissue-specific and developmentally important genes. Here, we generated 63,234 ESTs from our 5 constructed peanut cDNA libraries of Ralstonia solanacearum challenged roots, R. solanacearum challenged leaves, and unchallenged cultured peanut roots, leaves and developing seeds. Among these ESTs, there were 14,547 unique sequences with 7,961 tentative consensus sequences and 6,586 singletons. Putative functions for 47.8 % of the sequences were identified, including transcription factors, tissue-specific genes, genes involved in fatty acid biosynthesis and oil formation regulation, and resistance gene analogue genes. Additionally, differentially expressed genes, including those involved in ethylene and jasmonic acid signal transduction pathways, from both peanut leaves and roots, were identified in R. solanacearum challenged samples. This large expression dataset from different peanut tissues will be a valuable source for marker development and gene expression analysis. It will also be helpful for finding candidate genes for fatty acid synthesis and oil formation regulation as well as for studying mechanisms of interactions between the peanut host and R. solanacearum pathogen.