Prevalence of Escherichia coli O157:H7 on hides and faeces of ruminants at slaughter in two major abattoirs in Nigeria
Prevalence of Escherichia coli O157:H7 on hides and faeces of ruminants at slaughter in two major abattoirs in Nigeria
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DOI：
10.1111/j.1472-765x.2011.03113.x
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发表时间：
2011-09-01
影响因子：
2.4
通讯作者：
Kerry, P. C.
中科院分区：
文献类型：
--
作者：
Akanbi, B. O.;Mbah, I. P.;Kerry, P. C.
目的：确定大肠杆菌O 157：H7在尼日利亚屠宰反刍动物的皮和粪便中的发生率。方法和结果：从1月到12月，覆盖潮湿和哈麦丹季节，共采集了320头动物。在屠宰时从动物的皮和粪便中获得样品。ISO（ISO 16654：2001，食品和动物饲料的微生物学-检测大肠杆菌O 157的水平方法）富集和分离大肠杆菌的方法。coliO 157，采用改良胰蛋白胨大豆肉汤加新生霉素（mTSBn）选择性富集，免疫磁性分离，铺于含亚碲酸头孢克肟（CT-SMAC）的琼脂平板上。总体上，牛的患病率为49.4%，其次是绵羊和山羊，患病率分别为6.3%和2.5%。E.大肠杆菌O 157在两个不同牛种中的流行情况。大肠杆菌O 157：H7在国内两个屠宰场大量存在。研究了E.大肠杆菌O 157：H7不随季节而变化，但在热带国家的反刍动物群体和牛品种之间存在差异。研究的意义和影响：这是第一项关于大肠杆菌的研究。大肠杆菌O 157：H7。本研究强调了E.大肠杆菌O 157：H7沿着肉链传播，需要通过最佳卫生做法共同努力限制它。
Aim: To determine the occurrence of Escherichia coli O157: H7 in hides and faeces of slaughtered ruminants in Nigeria.Methods and Results: A total number of 320 animals were sampled from January to December covering the wet and harmattan seasons. Samples were obtained from the hides and faeces of animals at slaughter. The ISO (ISO 16654: 2001, Microbiology of food and animal feedingstuffs - horizontal method for the detection of Escherichia coli O157) method for enrichment and isolation of E. coli O157 incorporating selective enrichment using modified tryptone soya broth with novobiocin (mTSBn), immunomagnetic separation and plating on sorbitol-MacConkey agar with cefixime tellurite (CT-SMAC) was used. Overall cattle had a prevalence rate of 49.4% followed by sheep and goats with rates of 6.3% and 2.5%, respectively. There was a significant difference in carriage of E. coli O157 among two different cattle breeds.Conclusions: The prevalence of E. coli O157: H7 is substantial from two abattoirs in the country. The carriage and shedding of E. coli O157: H7 did not differ with season but differed among groups of ruminants and among breeds of cattle in a tropical country.Significance and Impact of the Study: This is the first study on E. coli O157: H7 from abattoir operations in Nigeria. The study emphasizes the risk of E. coli O157: H7 along the meat chain and the need for concerted effort to limit it through best hygiene practices.