Innervation of the Rat Uterus at Estrus: A Study In Full-Thickness, Immunoperoxidase-Stained Whole-Mount Preparations
Innervation of the Rat Uterus at Estrus: A Study In Full-Thickness, Immunoperoxidase-Stained Whole-Mount Preparations
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DOI：
10.1002/cne.22515
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发表时间：
2011-03-01
影响因子：
2.5
通讯作者：
Llewellyn-Smith, Ida J.
中科院分区：
文献类型：
--
作者：
Gnanamanickam, Greta J. E.;Llewellyn-Smith, Ida J.
在组织学切片中研究了非妊娠大鼠子宫的神经支配，其中仅包含少量神经样本，不太可能提供子宫神经支配的完整图像。在这里，我们使用了整个全厚子宫角的非妊娠大鼠在发情期的过氧化物酶免疫组织化学可视化自主神经或感觉神经的整装制剂。酪氨酸羟化酶（TH）标记的交感神经，囊泡乙酰胆碱转运蛋白（VAChT），副交感神经和P物质（SP）和降钙素基因相关肽（CGRP），感觉神经的免疫反应性进行了研究。神经肽Y（NPY）和一氧化氮合酶（NOS）确定了这些功能不同的神经类型之一以上。所有神经化学类的轴突进入子宫的子宫系膜和神经支配的子宫平滑肌。子宫线，一个致密带的纵向肌肉相对于子宫系膜，含有更多的TH-，NPY-，CGRP-，和VAChT-免疫反应性轴突比其余的平滑肌。NPY、SP、NOS和VAChT免疫反应阳性的轴突在环形肌-子宫内膜界面附近形成丛。罕见的TH和NPY免疫反应阳性轴突和偶尔CGRP免疫反应阳性轴突发生接近子宫腺。血管有密集的血管周围丛TH和NPY的轴突和较低密度的NOS，SP，CGRP和VAChT阳性丛。环肌丛和腺体在子宫系膜对面缺失。子宫小动脉在整个子宫内形成相互连接的网络。本文提供了第一个全面的描述的自主神经和感觉神经支配的非妊娠大鼠子宫，并将成为未来的研究基础，子宫神经支配的变化所造成的正常生理或病理生理的挑战J。519：621-643，2011。(C)2010 Wiley-Liss，Inc.
The innervation of the nonpregnant rat uterus has been studied in histological sections, which contain only small samples of nerves and are unlikely to afford a complete picture of uterine innervation. Here we used whole-mount preparations of entire full-thickness uterine horns from nonpregnant rats in estrus to visualize autonomic or sensory nerves with peroxidase immunohistochemistry. Immunoreactivity was studied for tyrosine hydroxylase (TH)-labeled sympathetic nerves; vesicular acetylcholine transporter (VAChT), parasympathetic nerves; and substance P (SP) and calcitonin gene-related peptide (CGRP), sensory nerves. Neuropeptide Y (NPY) and nitric oxide synthase (NOS) identified more than one of these functionally distinct nerve types. Axons of all neurochemical classes entered the uterus at the mesometrium and innervated the uterine smooth muscle. The linea uteri, a dense band of longitudinal muscle opposite the mesometrium, contained more TH-, NPY-, CGRP-, and VAChT-immunoreactive axons than the remaining smooth muscle. Axons immunoreactive for NPY, SP, NOS, and VAChT formed a plexus near the circular muscle-endometrium interface. Rare TH- and NPY-immunoreactive axons and occasional CGRP-immunoreactive axons occurred close to uterine glands. Blood vessels had dense perivascular plexuses of TH- and NPY-containing axons and less dense NOS-, SP-, CGRP-, and VAChT-positive plexuses. The circular muscle plexus and glands were absent opposite the mesometrium. Uterine arterioles formed an interconnected network throughout the uterus. This article provides the first comprehensive description of the autonomic and sensory innervation of the nonpregnant rat uterus and will be a foundation for future studies on changes in uterine innervation caused by normal physiological or pathophysiological challenges J. Comp. Neurol. 519:621-643, 2011. (C) 2010 Wiley-Liss, Inc.