Identification of alfonsino and related fish species belonging to the genus Beryx with mitochondrial 16S rRNA gene and its application on their pelagic eggs
Identification of alfonsino and related fish species belonging to the genus Beryx with mitochondrial 16S rRNA gene and its application on their pelagic eggs
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DOI：
10.1046/j.1444-2906.2002.00561.x
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发表时间：
2002-12-01
影响因子：
1.9
通讯作者：
Watabe, S
中科院分区：
文献类型：
--
作者：
Akimoto, S;Kinoshita, S;Watabe, S
以总DNA为模板，用聚合酶链反应扩增了alfonsino及其亲缘种Beryx、B. splendens、B. mollis和B. decadactylus的线粒体16S rRNA基因。而在不同种间，16S rRNA基因序列共包含492个核苷酸，分别在锦绣蓟与锦绣蓟、锦绣蓟与白杨蓟、锦绣蓟与白杨蓟之间存在3个、9个和8个种间变异。这些在伊豆群岛采集的具有Beryx属典型形态特征的远洋虫卵，根据线粒体16S rRNA基因的核苷酸序列，均被鉴定为B. splendens。
The mitochondrial 16S rRNA gene was amplified by polymerase chain reaction using total DNA as a template from muscle tissues of alfonsino and related fish species of alfonsino belonging to the genus Beryx, B. splendens, B. mollis and B. decadactylus. While the sequence of the 16S rRNA gene consisted of 492 nucleotides irrespective of the three species, three, nine and eight interspecific variations were shown between B. splendens and B. mollis, between B. splendens and B. decadactylus, and between B. mollis and B. decadactylus, respectively. The pelagic eggs, which were collected off Izu Islands and showed morphological characteristics typical to the genus Beryx were all identified as those of B. splendens from the nucleotide sequence of the mitochondrial 16S rRNA gene.