Effects of citric acid and fibronectin and laminin application in treating periodontitis.
Effects of citric acid and fibronectin and laminin application in treating periodontitis.
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柠檬酸、纤连蛋白和层粘连蛋白应用治疗牙周炎的效果。
DOI：
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发表时间：
1987
影响因子：
6.7
通讯作者：
W. Castelli
中科院分区：
文献类型：
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作者：
B. Smith;R. Caffesse;C. Nasjleti;S. Kon;W. Castelli
为了确定柠檬酸调节和纤连蛋白-层粘连蛋白应用在治疗自然发生的牙周炎中对新结缔组织附着的影响，使用了2只比格犬中每只的所有4个象限。每个象限包括：P2、P3、P4和M1牙齿。比较两种治疗方法在术后120 d组织学愈合反应的差异。治疗方法为：（1）手术（粘骨膜瓣）加柠檬酸;（2）手术加柠檬酸，然后应用纤连蛋白-层粘连蛋白。洁治和根面刨平后，冠状面和根面参考切迹被放置用于组织测量。每次随机分配治疗后，缝合皮瓣。处死后，将处理区域的组织块脱钙并连续切割，获得颊舌和近远中切片。使用Filar测微计测量颊侧5个距离：（1）从根面切迹到牙槽骨嵴;（2）从根面切迹到牙骨质冠侧范围;（3）从根面切迹到结合上皮顶端范围;（4）从游离龈缘到结合上皮顶端范围;（5）从冠侧切迹到牙槽骨嵴。结果显示，2种试验处理之间的5个测量值无差异。在近远中切片上，在分叉处进行表面积测定，评价新结缔组织（含或不含骨）或上皮占据的空间。为此，使用Zeiss IBAS图像分析系统将图像数字化，该系统具有4 mB的阵列处理器存储器，该阵列处理器存储器耦合到Newvicon TV摄像机和微计算机。（250字处删节）
To determine the effect on new connective tissue attachment of citric acid conditioning and fibronectin-laminin application in treating naturally occurring periodontitis, all 4 quadrants in each of 2 Beagle dogs were used. Each quadrant included: P2, P3, P4, and M1 teeth. 2 treatment modalities were employed and comparatively analyzed for differences in histological healing respponses at 120 days after surgery. The treatments were: (1) surgery (mucoperiosteal flaps) plus citric acid; (2) surgery plus citric acid followed by fibronectin-laminin application. After scaling and root planing, coronal and root surface reference notches were placed for histometric measurements. Following each of the randomly assigned treatments, flaps were sutured. After sacrifice, tissue blocks of treated areas were decalcified and serially cut, obtaining bucco-lingual and mesiodistal sections. Using a Filar micrometer, 5 distances were masured on the buccal aspect: (1) from root surface notch to alveolar bone crest; (2) from root surface notch to coronal extent of the cementum; (3) from root surface notch to apical extent of the junctional epithelium; 84) from free gingival margin to apical extent of junctional epithelium; (5) from the coronal notch to the alveolar bone crest. Results showed no differences among the 5 measurements between the 2 treatments tested. On mesio-distal sections, surface area determinations were made in the furcations, evaluating the space occupied by new connective tissue, with or without bone, or by epithelium. For this, images were digitized using a Zeiss IBAS Image analysis system with a 4mB of array processor memory coupled to a Newvicon TV camera and a microcomputer.(ABSTRACT TRUNCATED AT 250 WORDS)