Circulating Tumor Cells: Strategies for Capture, Analyses, and Propagation.
Circulating Tumor Cells: Strategies for Capture, Analyses, and Propagation.
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DOI：
10.1097/ppo.0000000000000310
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Cote RJ
中科院分区：
文献类型：
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作者：
Agarwal A;Balic M;El-Ashry D;Cote RJ
循环肿瘤细胞（CTC）在肿瘤扩散和转移中起着核心作用，最终导致大多数癌症死亡。允许从癌症患者血液中鉴定和计数罕见CTC的技术已经将CTC确立为癌症诊断和预后的重要临床生物标志物。事实上，目前对CTC以及肿瘤微环境的相关细胞（如循环癌相关成纤维细胞（cCAF））进行稳健表征的努力，有望揭示转移过程的关键见解。最终，一旦CTC能够可靠地培养和增殖为生物标本，用于癌症患者的精确管理和新疗法的发现，CTC的临床效用将完全实现。在这篇综述中，我们重点介绍了最新的CTC捕获和分析技术，并讨论了体外培养和繁殖CTC的策略。
Circulating tumor cells (CTC) play a central role in tumor dissemination and metastases, which are ultimately responsible for most cancer deaths. Technologies that allow for identification and enumeration of rare CTC from cancer patients’ blood have already established CTC as an important clinical biomarker for cancer diagnosis and prognosis. Indeed, current efforts to robustly characterize CTC as well as the associated cells of the tumor microenvironment such as circulating cancer associated fibroblasts (cCAF), are poised to unmask key insights into the metastatic process. Ultimately, the clinical utility of CTC will be fully realized once CTC can be reliably cultured and proliferated as a biospecimen for precision management of cancer patients, and for discovery of novel therapeutics. In this review, we highlight the latest CTC capture and analyses technologies, and discuss in vitro strategies for culturing and propagating CTC.