Foliar and root Cu supply affect differently Fe- and Zn-uptake and photosynthetic activity in soybean plants
Foliar and root Cu supply affect differently Fe- and Zn-uptake and photosynthetic activity in soybean plants
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DOI：
10.1016/j.envexpbot.2006.09.005
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发表时间：
2007-06-01
影响因子：
5.7
通讯作者：
Yruela, I.
中科院分区：
文献类型：
--
作者：
Bernal, M.;Cases, R.;Yruela, I.
比较研究了大豆叶片和根系处理对过量铜供应的影响。尽管两种处理都能在叶片中积累Cu，但在水培培养基中，叶片中Cu处理与补铜处理之间存在显著差异。与对照植株相比，施用50 μ M CuSO4提高了叶片叶绿素含量、类囊体析氧活性以及685和695 nm的荧光发射（735 nm）。与对照相比，在添加50 μ M CuSO4的水培培养基中生长的大豆植株叶绿素含量较低，类囊体的析氧活性降低，685和695 nm处的荧光发射减弱。另一方面，叶片元素分析显示两处理间存在差异。结果表明，在叶片过量施用Cu的植株中，Cu与铁的吸收没有拮抗作用，但在水培培养基中过量施用Cu的植株中，Cu与铁的吸收存在竞争关系。此外，还观察到锌吸收的差异。叶片Cu处理降低了锌含量，根部Cu处理则相反。有趣的是，叶片被Cu处理过的植物的表现与过量Cu存在下生长的细胞悬浮液相似。结果表明，与根细胞相比，叶片细胞对铜的吸收和转运途径不同。(c) 2006 Elsevier B.V.版权所有
A comparative study on the effect of excess copper (Cu) supplied through leaves and root treatments in soybean plants is presented. Although Cu was accumulated in leaves upon both treatments, important differences were observed between Cu treatment of leaves and Cu supplementation in hydroponic medium. The application of 50 mu M CuSO4 on leaves increased chlorophyll content, oxygen evolution activity of thylakoids and fluorescence emission at 685 and 695 nm with respect to emission at 735 nm compared with control plants. In contrast, soybean plants grown in hydroponic medium supplemented with 50 mu M CuSO4 exhibited lower chlorophyll content, decreased oxygen evolution activity of thylakoids and decreased fluorescence emission at 685 and 695 nm compared with control plants. On the other hand, the elemental analysis of leaves showed differences between the two treatments investigated. The results indicated that no antagonist interaction between Cu- and Fe-uptake took place in plants where excess Cu was applied on leaves but Cu compete with Fe-uptake in plants grown with excess Cu in the hydroponic medium. Furthermore, differences in Zn-uptake were also observed. Zn content decreased upon Cu treatment of leaves whereas the opposite was observed upon Cu treatment through roots. Interestingly, plants with Cu-treated leaves behaved similarly as cell suspensions grown in the presence of excess Cu. The results strongly indicate that different Cu-uptake and transport pathways must operate in leaf cells compared with root cells. (c) 2006 Elsevier B.V. All rights reserved.