Influence of the photothermal effect of a gold nanorod cluster on biofilm disinfection
Influence of the photothermal effect of a gold nanorod cluster on biofilm disinfection
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DOI：
10.1088/0957-4484/24/19/195104
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发表时间：
2013-05-17
期刊：
影响因子：
3.5
通讯作者：
Kim, Min Jun
中科院分区：
文献类型：
--
作者：
Jo, Wonjin;Kim, Min Jun
我们评估了一种利用金纳米棒簇的光热效应提高生物膜温度的生物膜消毒方法。金纳米棒 (GNR) 能够通过激光照射加热生物膜，产生足够的热量来裂解细菌。为了测试这一点，使用湿化学合成了 GNR，并使用光刻技术制造了单个 GNR 簇。 GNR 簇直接应用于生物膜，并在激光照射前后测量其对细菌的影响。 GNR 对生物膜结构的光热效应导致细胞活力和生物膜厚度显着降低。细菌死亡率和生物膜破坏的一些定量测量表明，随着激光照射持续时间的增加，功效也随之增加。受辐照细菌的扫描电镜图像显示出明显的形态损伤，例如生物膜中细菌细胞膜的破裂或塌陷。这些结果表明 GNR 是有用的，并且是用于光热处理（特别是生物膜消毒）的潜在材料。
We evaluate a method for biofilm disinfection by raising biofilm temperature using the photothermal effect of a gold nanorod cluster. Gold nanorods (GNRs) are capable of generating enough heat to lyse bacteria by heating biofilm via laser irradiation. To test this, GNRs are synthesized using wet chemistry and a single GNR cluster is fabricated using photo-lithography technique. The GNR cluster is directly applied to the biofilm and its effects on bacteria are measured before and after laser irradiation. The photothermal effect of GNRs on the biofilm structure results in a considerable reduction of cell viability and biofilm thickness. Several quantitative measurements of bacterial mortality and biofilm destruction show an increase in efficacy with increasing durations of laser irradiation. Scanning electron microscopy images of the irradiated bacteria show obvious morphological damage such as rupture or collapse of the bacterial cell membrane in the biofilm. These results indicate that GNRs are useful and a potential material for use in photothermal treatments, particularly biofilm disinfection.