The sensitivity of gross necropsy, caudal fold and comparative cervical tests for the diagnosis of bovine tuberculosis
The sensitivity of gross necropsy, caudal fold and comparative cervical tests for the diagnosis of bovine tuberculosis
复制标题
DOI：
10.1177/104063870401600206
复制
发表时间：
2004-03-01
影响因子：
1.5
通讯作者：
Payeur, JB
中科院分区：
文献类型：
--
作者：
Norby, B;Bartlett, PC;Payeur, JB
在密歇根州下半岛东北角的22个牛群中诊断出牛结核病（bTb）。在这22个畜群中，对7个畜群中的494只动物进行了大体尸检、组织病理学检查、分枝杆菌培养和聚合酶链反应（PCR）检测，这些检测仅对组织学上与bTb相容的样本进行。将平行解释的培养和PCR结果作为计算1)尾褶试验（CFT）、2)串联使用的尾褶和比较颈部皮肤试验（CFTCCTSER）和3)大体尸检敏感性的参考试验。从43只动物中分离到牛分枝杆菌。CFT、CFTCCTSER和大体尸检的敏感性分别为93.02%、88.37%和86.05%。按低、中流行人群分层时，低流行人群的敏感性分别为83.33%、75.0%和83.33%，中流行人群的敏感性分别为96.77%、93.55%和87.10%。当存在2个或更多的大体病变时，2项皮肤试验的敏感性略高，大体尸检的敏感性显著较高（P = 0.049）。发现CFT的敏感性明显高于其他研究中的大多数估计；然而，由于本研究中纯化蛋白衍生物的数量和参考方法与其他已发表的研究不同，因此无法进行直接比较。虽然敏感性很高，但如果使用了测试和移除程序，7个畜群中有2个（29%）将有1只或更多阳性动物留在畜群中。这表明，当确定呈阳性的畜群时，选择性扑杀皮肤试验反应器是一种比完全减少种群更不可接受的疾病控制策略。
Bovine tuberculosis (bTb) was diagnosed in 22 cattle herds in the northeast corner of Michigan's lower peninsula. Of these 22 herds, 494 animals in 7 herds were examined by gross necropsy, histopathologic exam, mycobacterial culture, and polymerase chain reaction (PCR) assay performed only on samples that were histologically compatible for bTb. Results of culture and PCR assay interpreted in parallel were used as the reference test for calculation of the sensitivity of 1) the caudal fold test (CFT), 2) the caudal fold and comparative cervical skin tests used in series (CFTCCTSER), and 3) gross necropsy. Mycobacterium bovis was isolated from 43 animals. Using all 7 herds, the sensitivities of the CFT, the CFTCCTSER and gross necropsy were 93.02%, 88.37%, and 86.05%, respectively. When the data were stratified by low- and moderate-prevalence herds, the sensitivities were 83.33%, 75.0%, and 83.33% in low-prevalence herds and 96.77%, 93.55%, and 87.10% in moderate-prevalence herds. The sensitivities of the 2 skin tests were slightly higher when 2 or more gross lesions were present, and the sensitivity of gross necropsy was significantly higher (P = 0.049). The sensitivity of the CFT was found to be notably higher than most estimates in other studies; however, a direct comparison was not possible because the amount of purified protein derivative and the reference methods were different in this study compared with other published studies. Although the sensitivities are high, 2 of the 7 herds (29%) would have had 1 or more positive animals left in the herd if a test-and-removal program had been used. This suggests that when positive herds are identified, selective culling of skin test reactors is a less acceptable disease control strategy than is complete depopulation.