Intraspecific genetic variation in Paramecium revealed by mitochondrial cytochrome c oxidase I sequences
Intraspecific genetic variation in Paramecium revealed by mitochondrial cytochrome c oxidase I sequences
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DOI：
10.1111/j.1550-7408.2005.00068.x
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发表时间：
2006-01-01
影响因子：
2.2
通讯作者：
Berendonk, TU
中科院分区：
文献类型：
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作者：
Barth, D;Krenek, S;Berendonk, TU
纤毛虫种内遗传多样性的研究，如种群遗传学和植物地理学的研究，由于缺乏合适的DNA标记而受到限制。例如，非编码核糖体内部转录间隔区（ITS）的序列往往过于保守，无法进行种内分析。因此，我们确定了线粒体细胞色素c氧化酶I（COI）基因的引物，并将其应用于草履虫尾草履虫和草履虫多微核的种内调查。此外，我们从相同的菌株中获得了ITS区的序列，并对两个数据集进行了比较序列分析。线粒体序列揭示了草履虫物种的变异显著较高，种内分歧高达7%的尾草履虫和9.5%的P.此外，人口结构的初步调查发现不同的线粒体单倍型的尾草履虫在一个池塘，从而证明了这种遗传标记的潜力，人口遗传分析。我们设计的引物成功地扩增出草履虫的COI基因。这是第一个报告的种内变异的自由生活的原生动物的线粒体序列数据的基础上。我们的研究结果表明，线粒体DNA的高度变异，使其成为一个合适的标记，种内和群体遗传研究。
Studies of intraspecific genetic diversity of ciliates, Such as population genetics and biogeography, are particularly hampered by the lack of suitable DNA markers. For example, sequences of the non-coding ribosomal internal transcribed spacer (ITS) regions are often too conserved for intraspecific analyses. We have therefore identified primers for the mitochondrial cytochrome c oxidase I (COI) gene and applied them for intraspecific investigations in Paramecium caudatum and Paramecium multimicronucleation. Furthermore, we obtained sequences of the ITS regions from the same strains and carried out comparative sequence analyses of both data sets. The mitochondrial sequences revealed substantially higher variation in both Paramecium species, with intraspecific divergences up to 7% in P. caudatum and 9.5% in P. Moreover, an initial survey of the population structure discovered different mitochondrial haplotypes of P. caudatum in one pond, thereby demonstrating the potential of this genetic marker for population genetic analyses. Our primers Successfully amplified the COI gene of other Paramecium. This is the first report of intraspecific variation in free-living protozoans based on mitochondrial sequence data. Our results show that the high variation in mitochondrial DNA makes it a suitable marker for intraspecific and population genetic Studies.