EVIDENCE OF HUMAN POLYOMAVIRUS-BK AND POLYOMAVIRUS-JC INFECTION IN NORMAL BRAIN-TISSUE
EVIDENCE OF HUMAN POLYOMAVIRUS-BK AND POLYOMAVIRUS-JC INFECTION IN NORMAL BRAIN-TISSUE
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DOI：
10.1016/0042-6822(92)90167-n
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发表时间：
1992-11-01
期刊：
影响因子：
3.7
通讯作者：
DORRIES, K
中科院分区：
文献类型：
--
作者：
ELSNER, C;DORRIES, K
多瘤病毒JC和BK感染在人群中普遍存在，JCV是唯一与中枢神经系统疾病进行性多灶性白质脑病相关的病毒。在试图分析多瘤病毒感染的发病机制时，我们询问了人类多瘤病毒是否在持续过程中侵入大脑。对67例PML以外疾病患者的脑尸检材料进行了多瘤病毒DNA检测。Southern blot分析显示，约20%的患者在健康脑组织中存在jcv特异性全长病毒基因组。特异型分析与聚合酶链反应和测序证实了这些数据。此外，检测到BKV DNA序列覆盖早期基因片段和控制区，两侧有早期和晚期蛋白质编码序列。从两个病例中克隆出的完整的BKV基因组支持了这样的假设，即在这些组织标本中不仅存在全长的JCV DNA，而且存在BKV基因组。所获得的数据表明，JCV和BKV多瘤病毒对大脑的双重感染是一种常见事件，并有力地证明这两种病毒经常建立潜伏的中枢神经系统感染。
Infection with the polyomaviruses JC and BK is ubiquitous in the human population and JCV is the only virus associated with the central nervous system disease progressive multifocal leukoencephalopathy. In the attempt to analyze the pathogenesis of polyomavirus infections we asked whether human polyomaviruses invade the brain during persistence. Brain autopsy material from 67 individuals with disorders other than PML was examined for the presence of polyomavirus DNA. Southern blot analysis demonstrated JCV-specific full-length virus genomes in healthy brain tissue in about 20% of the patients. Type-specific analysis with polymerase chain reaction and sequencing confirmed these data. Additionally, the presence of BKV DNA sequences covering an early gene fragment and the control region with flanking early and late protein coding sequences was detected. Cloning of the complete BKV genome from two cases supported the assumption that not only full-length JCV DNA was present in those tissue specimens but also BKV genomes. The data obtained demonstrate that dual infection of the brain with the polyomaviruses JCV and BKV is a common event and give strong evidence that both viruses frequently establish a latent CNS infection.