Improvements in glucose tolerance and insulin sensitivity after lifestyle intervention are related to changes in serum fatty acid profile and desaturase activities: the SLIM study
Improvements in glucose tolerance and insulin sensitivity after lifestyle intervention are related to changes in serum fatty acid profile and desaturase activities: the SLIM study
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DOI：
10.1007/s00125-006-0383-4
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发表时间：
2006-10-01
期刊：
影响因子：
8.2
通讯作者：
Blaak, E. E.
中科院分区：
文献类型：
--
作者：
Corpeleijn, E.;Feskens, E. J. M.;Blaak, E. E.
目的/假设：本研究的目的是调查生活方式干预引起的血清胆固醇酯脂肪酸谱和估计的去饱和酶活性的变化是否与2型糖尿病风险受试者胰岛素敏感性的改善有关。材料和方法：在马斯特里赫特生活方式干预和糖耐量受损研究（SLIM）中，97名患有IGT的男性和女性被随机分配到饮食和运动联合计划（47名干预）或对照组（50名对照受试者）。在基线和1年后进行以下评估：OGTT，确定最大有氧能力的运动试验，人体测量和血清胆固醇酯脂肪酸谱分析。结果生活方式方案能有效减少总脂肪和饱和脂肪的摄入，增加体力活动，减少肥胖，改善胰岛素敏感性和葡萄糖耐量。总体回归分析显示，C20: 4 n-6/C20:3 n-6比值（估计Delta 5-去饱和酶活性）的增加和C18:3 n-6/C18:2 n-6比值（估计Delta 6-去饱和酶活性）和C16:1 n-7/C16:0比值（估计Delta 9-去饱和酶活性或硬脂酰辅酶a去饱和酶-1）的降低与胰岛素抵抗的体内平衡模型评估的降低显著相关。在调整了生活方式后（体脂百分比、有氧能力和饱和脂肪摄入量的变化），这些关联部分减少，但仍具有统计学意义。结论/解释：生活方式引起的胆固醇酯脂肪酸谱和去饱和酶活性的变化与2型糖尿病风险受试者胰岛素敏感性的变化独立相关。
Aims/hypothesis: The aim of this study was to investigate whether lifestyle intervention-induced changes in serum fatty acid profile of cholesteryl esters and estimated desaturase activities are related to improvements in insulin sensitivity in subjects at risk of type 2 diabetes.Materials and methods: In the Study on Lifestyle Intervention and Impaired Glucose Tolerance Maastricht (SLIM), 97 men and women with IGT were randomised to a combined diet and exercise programme (47 intervention) or a control group (50 control subjects). At baseline and after 1 year the following assessments were made: an OGTT, an exercise test to determine maximal aerobic capacity, anthropometry, and analysis of the serum fatty acid profile of cholesteryl esters.Results The lifestyle programme was effective in reducing the intake of total and saturated fat, increasing physical activity, reducing obesity and improving insulin sensitivity and glucose tolerance. Regression analysis of the total population showed that an increase in the C20: 4 n-6/C20:3 n-6 ratio (estimated Delta 5-desaturase activity) and reductions in the C18:3 n-6/C18:2 n-6 ratio (estimated Delta 6-desaturase activity) and the C16:1 n-7/C16:0 ratio (estimated Delta 9-desaturase activity or stearoyl-CoA desaturase-1) were significantly associated with a decrease in homeostasis model assessment for insulin resistance. After adjustment for lifestyle changes (change in percentage body fat, aerobic capacity and saturated fat intake), these associations were partly reduced, but remained statistically significant.Conclusions/interpretation: Lifestyle-induced changes in fatty acid profile of cholesteryl esters and desaturase activities were independently related to changes in insulin sensitivity in subjects at risk of type 2 diabetes.