Notch-signalling is required for head regeneration and tentacle patterning in Hydra
Notch-signalling is required for head regeneration and tentacle patterning in Hydra
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DOI：
10.1016/j.ydbio.2013.08.022
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发表时间：
2013-11-01
影响因子：
2.7
通讯作者：
Boettger, Angelika
中科院分区：
文献类型：
--
作者：
Muender, Sandra;Tischer, Susanne;Boettger, Angelika
局部自激活和长期抑制提供了一种机制，用于建立组织区域作为周围组织中结构发育的信号中心。成年水螅的下体起着头部组织者的作用。去除九头蛇头后，它是新形成的，完整的头可以再生。该组织区域的分子成分涉及Wnt信号传导和β-连环蛋白。然而，目前尚不清楚如何在水螅头中实现hypostome和触角的正确图案化，以及是否需要除了HyWnt 3之外的其他信号来在头移除后重新建立新的组织器。在这里，我们表明，缺口信号需要重新建立组织者在再生过程中，这是由于它的作用，限制肌腱激活。阻断Notch信号传导导致不规则头部结构的形成，其特征在于过多的腱组织和标记腱边界的基因的异常表达。这表明Notch信号在定义水螅头中的张力模式中的作用。此外，HvNotch及其靶HyHes的侧向抑制是头部再生所需的，如果没有这种抑制，β-连环蛋白/Wnt依赖性头部组织者的形成就会受损。对prebilaterian模式生物的研究表明，Wnt通路对于在早期多细胞动物中建立轴向模式的信号中心非常重要。我们的数据表明，Wnt信号与Notch-Delta活性的整合也参与了动物定义的身体计划的进化。(C)2013 Elsevier Inc. All rights reserved.
Local self-activation and long ranging inhibition provide a mechanism for setting up organising regions as signalling centres for the development of structures in the surrounding tissue. The adult hydra hypostome functions as head organiser. After hydra head removal it is newly formed and complete heads can be regenerated. The molecular components of this organising region involve Wnt-signalling and beta-catenin. However, it is not known how correct patterning of hypostome and tentacles are achieved in the hydra head and whether other signals in addition to HyWnt3 are needed for re-establishing the new organiser after head removal. Here we show that Notch-signalling is required for re-establishing the organiser during regeneration and that this is due to its role in restricting tentacle activation. Blocking Notch-signalling leads to the formation of irregular head structures characterised by excess tentacle tissue and aberrant expression of genes that mark the tentacle boundaries. This indicates a role for Notch-signalling in defining the tentacle pattern in the hydra head. Moreover, lateral inhibition by HvNotch and its target HyHes are required for head regeneration and without this the formation of the beta-catenin/Wnt dependent head organiser is impaired. Work on prebilaterian model organisms has shown that the Wnt-pathway is important for setting up signalling centres for axial patterning in early multicellular animals. Our data suggest that the integration of Wnt-signalling with Notch-Delta activity was also involved in the evolution of defined body plans in animals. (C) 2013 Elsevier Inc. All rights reserved.