Molecular Characterization of Myostatin Gene from Zhikong scallop Chlamys farreri (Jones et Preston 1904)
Molecular Characterization of Myostatin Gene from Zhikong scallop Chlamys farreri (Jones et Preston 1904)
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直孔扇贝 Chlamys farreri 的肌肉生长抑制素基因的分子特征 (Jones et Preston 1904)
DOI：
10.1266/ggs.85.207
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发表时间：
2010-06-01
影响因子：
1.1
通讯作者：
Bao, Zhenmin
中科院分区：
文献类型：
--
作者：
Hu, Xiaoli;Guo, Huihui;Bao, Zhenmin
扇贝(Chlamys Farreri Jones Et Preston 1904)是一种具有重要经济价值的海产品，其内收肌大而美味。研究扇贝肌肉生长的分子基础对扇贝育种和了解肌肉质量调节具有重要意义。肌肉生长抑制素(Myostatin，MSTN)是一种保守的肌肉生长发育负调控因子。报道了栉孔扇贝(Chlamys Farreri Jones Et Preston 1904)的MSTN基因，该基因由11651个核苷酸组成，编码457个氨基酸，该基因具有一个与脊椎动物同源基因保守的3-外显子/2-内含子结构。经5086和1518个核苷酸的内含子分离，该蛋白质序列含有一个蛋白降解切割基序(RXXR)和9个半胱氨酸等特征保守残基。在翻译起始密码子ATG的上游62、146和296个碱基上发现了3个转录起始点。通过对启动子区域的电子分析，发现了一个TATA-box和几个肌肉特异性调控元件，包括comp、mef2、MTBF和E-box。MSTN基因定位于一对中端着丝粒染色体的长臂，对胚胎/幼体MSTN转录本的定量分析表明，MSTN在原肠胚中高表达，在成体扇贝其他时期表达有限，MSTN主要在横纹肌中表达，在其他组织中表达水平不同。本研究为进一步研究扇贝MSTN的功能和进化提供了有价值的基因组和表达信息
The scallop is an economically important sea food prized for its large and delicious adductor muscle Studying the molecular basis of scallop muscle growth is important for both scallop breeding and our understanding of muscle mass regulation in bivalve Myostatin (MSTN) is a conserved negative regulator of muscle growth and development Here we report the MSTN gene from Zhikong scallop (Chlamys farreri Jones et Preston 1904) The C farreri MSTN consists of 11651 nucleotides encoding 457 amino acids The gene has a 3-exon/2-intron structure that is conserved with vertebrate homologs The exons are 586, 380 and 408 bp in length, respectively, and separated by introns of 5086 and 1518 bp The protein sequence contains characteristic conserved residues including a cleavage motif of proteolysis (RXXR) and nine cysteines Three transcription initiation sites were found at 62, 146, and 296 bp upstream of the translation start codon ATG In silico analysis of the promoter region identified a TATA-box and several muscle-specific regulatory elements including COMP, MEF2s, MTBFs and E-boxes Minisatellite DNA was found in intron 1 By fluorescence in situ hybridization (FISH), the gene was mapped to the long arm of a pair of middle subtelocentric chromosome Quantitative analysis of MSTN transcripts in embryos/ larvae indicated high expression level in gastrulae and limited expression at other stages In adult scallops, MSTN is predominantly expressed in striated muscle, with different expression levels in other tissues Our data provide valuable genomic and expression information which will aid the further study on scallop MSTN function and MSTN evolution