Direct Effect of Intracanal Medicaments on Survival of Stem Cells of the Apical Papilla
Direct Effect of Intracanal Medicaments on Survival of Stem Cells of the Apical Papilla
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DOI：
10.1016/j.joen.2012.06.018
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发表时间：
2012-10-01
影响因子：
4.2
通讯作者：
Diogenes, Anibal
中科院分区：
文献类型：
--
作者：
Ruparel, Nikita B.;Teixeira, Fabricio B.;Diogenes, Anibal
再生牙髓治疗是治疗牙髓坏死的一种替代方法。通常使用肛门内药物，如三重抗生素膏（TAP）或双重抗生素膏（DAP）和氢氧化钙（Ca[OH](2)）进行消毒。然而，它们对人根尖乳头（SCAPs）干细胞的影响尚不清楚。我们假设高浓度的肛管内药物对SCAPs有毒性。为了验证这一假设，使用了细胞培养试验。方法：简单地说，将SCAPs培养并进行无药物处理或不同浓度的处理，包括TAP、DAP、改性TAP（环丙沙星、甲硝唑和头孢克洛）、Augmentin （Champs Pharmacy, San Antonio， TX）或Ca(OH)(2)。在治疗后3天，使用自动检测台盼蓝染料的方法获得活干细胞计数。结果：所有4种抗生素均以浓度依赖的方式显著降低SCAP生存率。有趣的是，Ca(OH)(2)在所有浓度下都有利于SCAP的存活。结论：总的来说，我们的数据表明高浓度抗生素对SCAP的生存有不利影响，而低浓度以及所有测试浓度的Ca(OH)(2)有利于SCAP的生存和增殖。这些研究强调了临床上重要的一点，即必须在对干细胞活力影响最小的情况下，以杀菌浓度使用肛管内药物。[J] end2012;38:1372-1375 .]
Introduction: Regenerative endodontic procedures are an alternative treatment for immature teeth with necrotic pulps. Typically, intracanal medicaments such as triple antibiotic paste (TAP) or double antibiotic paste (DAP) and calcium hydroxide (Ca[OH](2)) are used for disinfection. However, their effect on human stem cells of the apical papilla (SCAPs) is unknown. We hypothesized that intracanal medicaments at high concentrations are toxic to SCAPs. To test this hypothesis, a cell culture assay was used. Methods: Briefly, SCAPs were cultured and subjected to either no drug treatment or various concentrations including TAP, DAP, modified TAP (ciprofloxacin, metronidazole and cefaclor), Augmentin (Champs Pharmacy, San Antonio, TX), or Ca(OH)(2). Viable stem cells counts were obtained using an automated method of detecting trypan blue dye at 3 days after treatment. Results: All 4 antibiotics significantly reduced SCAP survival in a concentration-dependent fashion. Interestingly, Ca(OH)(2) was conducive with SCAP survival at all concentrations. Conclusions: Collectively, our data show that high concentrations of antibiotics have a detrimental effect on SCAP survival, whereas lower concentrations as well as Ca(OH)(2) at all tested concentrations are conducive with SCAP survival and proliferation. These studies highlight the clinically important point that intracanal medicaments must be used at concentrations that are bactericidal while having minimal effects on stem cell viability. (J Endod 2012;38:1372-1375)