Development of Antimicrobial Thermoplastic Material from Archaeal Poly-γ-L-Glutamate and Its Nanofabrication
Development of Antimicrobial Thermoplastic Material from Archaeal Poly-γ-L-Glutamate and Its Nanofabrication
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DOI：
10.1021/am3032025
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发表时间：
2013-03-13
影响因子：
9.5
通讯作者：
Kitagawa, Masaru
中科院分区：
文献类型：
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作者：
Ashiuchi, Makoto;Fukushima, Kenzo;Kitagawa, Masaru
本文描述了一种古细菌聚γ - l -谷氨酸（L-PGA）和十六烷基吡啶阳离子（HDP+）的化学测量离子配合物，称为pgac，它具有出色的耐化学性和作为新型功能热塑性塑料的潜力。pgac薄膜抑制原核生物（大肠杆菌、枯草芽孢杆菌、鼠伤寒沙门氏菌和金黄色葡萄球菌）和真核生物（酿酒酵母）微生物的增殖。此外，其抗真菌活性被证明对一种流行的念珠菌（白色念珠菌）和一种丝状真菌（黑曲霉）。估计最低抑制浓度为0.25 mg mL(-1)，当pgaic涂层的聚对苯二甲酸乙二醇酯（PET）薄膜放置在培养板上时，出现生长抑制区，而PET对真菌生长的影响很小。因此，可溶性PGAIC有望作为一种抗菌剂和涂层底物。我们还成功地用pgac的乙醇溶液合成了l - pgac基纳米纤维。
Here we describe a stoichiometric ion-complex of archaeal poly-gamma-L-glutamate (L-PGA) and hexadecylpyridinium cation (HDP+), called PGAIC, which shows remarkable chemical resistance and potential as a novel functional thermoplastic. PGAIC films suppressed the proliferation of prokaryotic (Escherichia coli, Bacillus subtilis, Salmonella typhimurium, and Staphylococcus aureus) and eukaryotic (Saccharomyces cerevisiae) microorganisms. Moreover, its antifungal activity was demonstrated against a prevalent species of Candida (Candida albicans) and a filamentous fungus (Aspergillus niger). The minimal inhibitory concentrations were estimated as 0.25 mg mL(-1), and zones of growth inhibition appeared when PGAIC-coated polyethylene terephthalate (PET) films were placed in culture plates, whereas PET had very little effect on fungal growth. Soluble PGAIC thus shows promises as an antimicrobial and as a coating substrate. We also succeeded in synthesizing an L-PGA-based nanofiber using an ethanol solution of PGAIC.