Specific progesterone receptors in the hypothalamus and anterior hypophysis of the rat.
Specific progesterone receptors in the hypothalamus and anterior hypophysis of the rat.
复制标题
大鼠下丘脑和前垂体中的特异性孕酮受体。
作者：
Junzo Kato;T. Onouchi
雌激素致敏的未成熟和成熟雌性大鼠的下丘脑和前垂体细胞质中检测到孕酮结合成分。这些成分在体外标记的氚化合成的孕激素，R5020，特异性结合孕酮受体。放射性复合物在含有10%甘油的蔗糖密度梯度中沉淀在7S区域中。在大脑皮层、杏仁核或网状结构的类似制剂中检测到很少的7S结合。与雌激素引发的显着效果相反，在完整的nonprimed未成熟雌性大鼠的下丘脑和垂体中发现非常低或没有7S结合。未成年大鼠下丘脑组分的解离常数（Kd）和结合位点数（NBS）分别为3.3 × 10− 9 M和1.0 × 10−14 mol/mg胞浆蛋白。成年大鼠前垂体的这些值分别为3.8 × 10− 9 M和3.1 × 10−14 mol/mg胞质溶胶蛋白。7S绑定组件.
Progesterone-binding components were detected in hypothalamic and anterior hypophysial cytosols from estrogen-primed immature and mature female rats. These components were labeled in vitro with a tritiated synthetic progestin, R5020, which binds specifically to progesterone receptors. The radioactive complex sediments in the 7S region in sucrose density gradients containing 10% glycerol. Little 7S binding was detected in similar preparations from the cerebral cortex, amygdala or reticular formation. In contrast with the marked effects by the estrogen-priming, very low or no 7S binding was found in the hypothalamus and hypophysis from intact nonprimed immature female rats. The dissociation constants (Kd) and the number of binding sites (NBS) of the hypothalamic components in immature rats were 3.3 × 10−9M and 1.0 × 10−14 moles\mg cytosol protein, respectively. These values for the adult rat anterior hypophysis were 3.8 × 10−9M and 3.1 × 10−14 moles/mg cytosol protein, respectively. The 7S binding component...