Tumor necrosis factor-α and transforming growth factor-β1 facilitate differentiation and proliferation of tendon-derived stem cells in vitro
Tumor necrosis factor-α and transforming growth factor-β1 facilitate differentiation and proliferation of tendon-derived stem cells in vitro
复制标题
肿瘤坏死因子-α和转化生长因子-β1促进肌腱干细胞体外分化和增殖
DOI：
10.1007/s10529-017-2296-3
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发表时间：
2017-05-01
影响因子：
2.7
通讯作者：
Li, Zhaozhu
中科院分区：
文献类型：
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作者：
Han, Peilin;Cui, Qingbo;Li, Zhaozhu
目的探讨肿瘤坏死因子-α（TNF-α）在肿瘤细胞中的作用（TNF-α）和转化生长因子-β 1结果TNF-α抑制肌腱源性干细胞（TDSC）的增殖和成腱/成骨分化，但同时或序贯应用TGF-β 1和TNF-α后，成腱/成骨相关标志物的表达和TDSC的增殖显著增加。在这些过程中，Smad 2/3和Smad 1/5/8被高度磷酸化，这意味着TGF-β和BMP信号通路被高度激活。结论TNF-α和TGF-β 1联合应用可促进TDSC的增殖和分化，且I-Smad的表达与TDSC的增殖和分化呈负相关。
Objectives To investigate the effects of tumor necrosis factor-alpha (TNF- alpha) and transforming growth factor-beta 1 (TGF- beta 1) on the proliferation and differentiation of tendon-derived stem cells (TDSC).Results TNF- alpha inhibits the proliferation and tenogenic/ osteogenic differentiation of TDSC but, after simultaneous or sequential treatment with TGF- beta 1 and TNF- alpha, the expression of tenogenic/osteogenic-related marker and proliferation of TDSC was significantly increased. During these processes, Smad2/3 and Smad1/5/8 were highly phosphorylated, meaning that the TGF- beta and BMP signaling pathways were highly activated. Further study revealed that the expression of Inhibitor-Smad appeared to be negatively correlated to the proliferation and differentiation of TDSC.Conclusions Combining the use of TNF- alpha and TGF beta 1 could improve the proliferation and differentiation of TDSC in vitro, and the expression of I-Smad is negatively correlated with TDSC proliferation and differentiation.