Receptor-targeted optical imaging of tumors with near-infrared fluorescent ligands
Receptor-targeted optical imaging of tumors with near-infrared fluorescent ligands
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DOI：
10.1038/86707
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发表时间：
2001-04-01
影响因子：
46.9
通讯作者：
Grötzinger, C
中科院分区：
文献类型：
--
作者：
Becker, A;Hessenius, C;Grötzinger, C
在这里，我们报告了在体内诊断使用的肽染料共轭物组成的花青染料和生长抑素类似物奥曲酸作为光学肿瘤成像的造影剂。当用于小鼠异种移植物的全身体内成像时，吲哚三碳菁-奥曲肽在肿瘤组织中积累。应用后3 ~ 24 h，肿瘤荧光迅速增加，是正常组织荧光的3倍以上。靶向缀合物也被原代人神经内分泌肿瘤细胞特异性内化。这种成像方法结合了配体/受体相互作用的特异性和近红外荧光检测，可以应用于癌症诊断的各种其他领域。
We report here the in vivo diagnostic use of a peptide-dye conjugate consisting of a cyanine dye and the somatostatin analog octreotate as a contrast agent for optical tumor imaging. When used in whole-body in vivo imaging of mouse xenografts, indotricarbocyanine-octreotate accumulated in tumor tissue. Tumor fluorescence rapidly increased and was more than threefold higher than that of normal tissue from 3 to 24 h after application. The targeting conjugate was also specifically internalized by primary human neuroendocrine tumor cells. This imaging approach, combining the specificity of ligand/receptor interaction with near-infrared fluorescence detection, may be applied in various other fields of cancer diagnosis.