MICROBIAL TRANSFORMATION OF STEROLS .I. DECOMPOSITION OF CHOLESTEROL BY MICROORGANISMS
MICROBIAL TRANSFORMATION OF STEROLS .I. DECOMPOSITION OF CHOLESTEROL BY MICROORGANISMS
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DOI：
10.1080/00021369.1969.10859516
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发表时间：
1969-01-01
期刊：
影响因子：
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通讯作者：
TAMURA, G
中科院分区：
文献类型：
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作者：
ARIMA, K;NAGASAWA, M;TAMURA, G
检测了1589株微生物的胆固醇分解能力。发现来自放线菌、细菌、霉菌和酵母的两百三十六种菌株能够将胆固醇氧化成胆烯酮。 Cholesta-1,4-dien-3-one 由 5 株链霉菌产生。在以下属中观察到胆固醇分子的完全分解：节杆菌属、芽孢杆菌属、短杆菌属、棒状杆菌属、微杆菌属、分枝杆菌属、诺卡氏菌属、精蛋白杆菌属、沙雷氏菌属和链霉菌属。 α,α'-联吡啶和亚砷酸盐抑制分解酶，产生胆烯酮、cholesta-1,4-dien-3-one 和可能不含甾醇侧链的中间体。在存在的情况下，各种甾醇的 C-17 侧链选择性断裂，产生雄甾烯-1,4-二烯-3,17-二酮 (ADD)由以下属的微生物产生α，α'-联吡啶：节杆菌属、芽孢杆菌属、短杆菌属、棒杆菌属、微杆菌属、分枝杆菌属、诺卡氏菌属、精蛋白杆菌属、沙雷氏菌属和链霉菌属。以胆固醇为例，其降解途径如下：其他甾醇如菜油甾醇、β-谷甾醇、豆甾醇和7-脱氢胆固醇均按相同的序列降解。以胆固醇为例的途径被认为是甾醇通过其分解微生物的一般降解途径。进一步证明，由甾醇形成的ADD被转化为3-羟基-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione。
Cholesterol decomposing ability of 1589 microbial strains was examined. Two hundreds and thirty six strains from actinomycetes, bacteria, molds, and yeasts were found capable of oxidizing cholesterol into cholestenone. Cholesta-1,4-dien-3-one was produced by 5 strains ofStreptomyces. The complete decomposition of cholesterol molecule was observed in the genera:Arthrobacter, Bacillus, Brevibacterium, Corynebacterium, Microbacterium, Mycobacterium, Nocardia, Protaminobacter, Serratia, andStreptomyces. α,α′-Dipyridyl and arsenite inhibited decomposing enzymes giving rise to cholestenone, cholesta-1,4-dien-3-one, and an intermediate probably devoid of the sterol side chain.Selective cleavage of the side chains of various sterols at C-17, giving rise to androsta-1,4-diene-3,17-dione (ADD), occurred in the presence ofα,α′-dipyridyl by microorganisms of the following genera:Arthrobacter,Bacillus,Brevibacterium,Corynebacterium, Microbacterium,Mycobacterium,Nocardia, Protaminobacter, Serratia, andStreptomyces. The degradation pathway of cholesterol, for example, was shown as follows:Other sterols such as campesterol,β-sitosterol, stigmasterol and 7-dehydrocholesterol were degraded by the same sequence. The pathway exemplified in cholesterol is considered to be the general degradation pathway of sterols by their decomposing microorganisms.It was further demonstrated that ADD thus formed from sterols was converted into 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione.