Capillary electrophoresis coupled to inductively coupled plasma mass spectrometry (CE/ICP-MS) and to electrospray ionization mass spectrometry (CE/ESI-MS): An approach for maximum species information in speciation of selenium
Capillary electrophoresis coupled to inductively coupled plasma mass spectrometry (CE/ICP-MS) and to electrospray ionization mass spectrometry (CE/ESI-MS): An approach for maximum species information in speciation of selenium
复制标题
毛细管电泳与电感耦合等离子体质谱 (CE/ICP-MS) 和电喷雾电离质谱 (CE/ESI-MS) 联用：获取硒形态中最大物种信息的方法
DOI：
10.1007/s002160051222
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
A. Kettrup
中科院分区：
文献类型：
--
作者：
B. Michalke;O. Schramel;A. Kettrup
由分离和检测步骤组成的联机联用技术是鉴定和表征含金属物种的最有效的技术之一。毛细管电泳（CE）的高分辨能力被用于三种硒形态的分离，而电喷雾电离质谱（ESI-MS）和电感耦合等离子体质谱（ICP-MS）被用于分子或元素的特异性检测，本文综述了这两种联用系统在硒形态研究中的可能性和局限性。以5%乙酸为背景电解质的毛细管电泳法分离硒代蛋氨酸（SeM）、硒代胱氨酸（SeC）和硒代胱胺（SeCM），结果表明，与CE/ESI-MS联用系统相比，CE/ICP-MS联用系统的检出限提高了102 ~ 103倍。
Abstract On-line hyphenations consisting of a separation and a detection step are one of the most efficient techniques for identification and characterization of metal containing species. The high resolution power of capillary electrophoresis (CE) is used for the separation of three selenium species, whereas either electrospray ionization mass spectrometry (ESI-MS) or inductively coupled plasma mass spectrometry (ICP-MS) are taken for molecule or element specific detection.This work gives an overview about the possibilities and limitations, when using the two hyphenated systems for speciation investigations. In order to show the power of the two complementary techniques, a CZE method using 5% acetic acid as background electrolyte was applied to the separation of selenomethionine (SeM), selenocystine (SeC) and selenocystamine (SeCM).Depending on the species and the element, the detection limits of the CE/ICP-MS hyphenated system are up to 102 to 103 times better than that for the CE/ESI-MS system.