Remodeling of the Photosynthetic Chain Promotes Direct CO2 Conversion into Valuable Aromatic Compounds
Remodeling of the Photosynthetic Chain Promotes Direct CO2 Conversion into Valuable Aromatic Compounds
复制标题
光合链的重塑促进二氧化碳直接转化为有价值的芳香化合物
DOI：
10.1002/anie.201808402
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发表时间：
2018-12-03
影响因子：
16.6
通讯作者：
Xu, Ping
中科院分区：
文献类型：
--
作者：
Ni, Jun;Liu, Hong-Yu;Xu, Ping
利用光合作用微生物指导二氧化碳转化提供了一条将二氧化碳封存与石化置换相结合的有希望的途径。然而，低通量的莽草酸途径在很大程度上仍未被用于合成有价值的芳香族化合物。此外，目前还不清楚增强的低通量途径将如何影响光合链。我们通过在细长聚球藻中引入2-苯乙醇途径和人工抗反馈抑制盒，创造了一个强大的代谢汇。超过30%的固定碳被重定向到莽草酸合成芳香族的途径，固碳和放氧显著增加。发现了光合链的自我重塑机制，它加速了电子传递，减少了能量浪费。这项研究代表着将二氧化碳转化为芳香族化合物的工业可行性迈出了重要的一步，并为提高光合作用效率提供了设计指导。
Directing CO2 conversion using photosynthetic microorganisms offers a promising route to couple CO2 sequestration with petrochemical replacement. However, the low-flux shikimate pathway remains largely unexploited for the synthesis of valuable aromatics. In addition, it is unclear how an enhanced low-flux pathway would influence the photosynthetic chain. We created a powerful metabolic sink by introducing the 2-phenylethanol pathway and an artificial feedback-inhibition-resistant cassette to Synechococcus elongatus. More than 30% of the fixed carbon was redirected to the shikimate pathway for aromatic synthesis, and carbon fixation and O-2 evolution increased significantly. A self-remodeling mechanism of the photosynthetic chain was discovered, which accelerates electron transport and reduces energy waste. This study represents a significant step toward the industrial viability of CO2 conversion into aromatic compounds and provides design guidance for improving photosynthetic efficiency.