Cloning and sequence analysis of a cDNA for a rat liver glutathione S-transferase Yb subunit.
Cloning and sequence analysis of a cDNA for a rat liver glutathione S-transferase Yb subunit.
复制标题
大鼠肝脏谷胱甘肽 S-转移酶 Yb 亚基 cDNA 的克隆和序列分析。
DOI：
10.1093/nar/14.15.6101
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发表时间：
1986
影响因子：
14.9
通讯作者：
Tu,CP
中科院分区：
文献类型：
--
作者：
Lai,HC;Grove,G;Tu,CP
我们从大鼠肝多聚核糖体多聚（A）RNA制备的谷胱甘肽S-转移酶（GST）cDNA文库中分离到Yb亚基cDNA克隆。对这些cDNA之一pGTR 200的序列分析揭示了218个氨基酸的开放阅读框架，Mr= 25，915。推导的序列与GST-A的19个氨基端残基一致。pGTR 200的序列与另一个YbcDNA，pGTA/C44的编码区中的4个核苷酸和2个氨基酸不同，从而揭示了序列的微异质性。pGTR 200中的cDNA插入片段也含有36个核苷酸的5′端非编码区和一个完整的3′端非编码区。Yb亚基cDNA与Yaor YccDNA的同源性非常有限，但与胎盘亚基Ypclone pGP 5具有相对较高的序列同源性。pGTR 200的mRNA在大鼠心脏和精囊中不大量表达。因此，pGTR 200的GST亚基序列可能代表一个基本的Yb亚基。基因组DNA杂交模式显示了一个复杂性，与Ybsubunlts的多基因家族一致。Ya、Yb、Yc和Yp亚基的氨基酸序列的比较显示，在整个编码序列中氨基酸具有显著的保守性（约29%）。这些结果表明，大鼠GST是至少四个不同基因的产物，可能构成一个超基因家族。
We have isolated a Yb-subunit cDNA clone from a GSH S-transferase (GST) cDNA library made from rat liver polysomal poly(A) RNAs. Sequence analysis of one of these cDNA, pGTR200, revealed an open reading frame of 218 amino acids of Mr=25,915. The deduced sequence is in agreement with the 19 NH2-terminal residues for GST-A. The sequence of pGTR200 differs from another YbcDNA, pGTA/C44 by four nucleotides and two amino acids in the coding region, thus revealing sequence microheterogeneity. The cDNA insert in pGTR200 also contains 36 nucleotides in the 5′ noncoding region and a complete 3′ noncoding region. The Ybsubunit cDNA shares very limited homology with those of the Yaor YccDNAs, but has relatively higher sequence homology to the placental subunit Ypclone pGP5. The mRNA of pGTR200 is not expressed abundantly in rat hearts and seminal vesicles. Therefore, the GST subunit sequence of pGTR200 probably represents a basic Ybsubunit. Genomic DNA hybridization patterns showed a complexity consistent with having a multigene family for Ybsubunlts. Comparison of the amino acid sequences of the Ya, Yb, Yc, and Ypsubunits revealed significant conservation of amino acids (∼ 29%) throughout the coding sequences. These results indicate that the rat GSTs are products of at least four different genes that may constitute a supergene family.