Expression of nitric oxide synthase by syncytiotrophoblast in human placental villi.
Expression of nitric oxide synthase by syncytiotrophoblast in human placental villi.
复制标题
人胎盘绒毛中合体滋养层一氧化氮合酶的表达。
DOI：
10.1096/fasebj.7.13.7691671
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Davis,AK
中科院分区：
文献类型：
--
作者：
Conrad,KP;Vill,M;McGuire,PG;Dail,WG;Davis,AK
一氧化氮（NO）的内源性生物合成在妊娠期间增加。为了开始我们对可能的组织来源的调查，我们检查了胎盘。我们推测，类似于血管内皮细胞，人胎盘绒毛间血隙的合胞体滋养层（STr）细胞层将表达NO合酶。我们的研究结果表明，人胎盘绒毛表达一种钙和钙调素敏感形式的NO合酶，主要位于微粒体细胞组分中。通过使用从人内皮NO合酶cDNA产生的核糖核酸探针进行原位杂交，我们观察到STr中NO合酶mRNA的表达。STr还显示NADPH-黄递酶染色，表明存在NO合酶，并且很可能存在参与性类固醇代谢的其他含黄素的酶。一氧化氮合酶活性也检测到绒毛的一个完整的痣胎盘（其中缺乏胎儿血管），进一步支持滋养层起源。我们的研究结果表明，STr衍生的NO在胎盘功能中的作用以前未被认识。康拉德，K。P.，Villi，M.，McGuire，P. G.，Dail，W. G.，Davis，A. K.一氧化氮合酶在人类胎盘绒毛中的合胞滋养层表达。FASEB J.7：1269 - 1276; 1993。
The endogenous biosynthesis of nitric oxide (NO) is increased during gestation. To begin our investigation of a possible tissue source (or sources), we examined the placenta. We postulated that analogous to the endothelium of blood vessels, the syncytiotrophoblast (STr) cell layer that lines the intervillous blood space of the human placenta would express NO synthase. Our results show that human placental villi express a calcium‐ and calmodulin‐sensitive form of NO synthase, located mainly in the microsomal cell fraction. By in situ hybridization using a riboprobe generated from human endothelial NO synthase cDNA, we observe NO synthase mRNA expression in STr. The STr also shows NADPH‐diaphorase staining, indicating the presence of NO synthase, and most likely other flavin‐containing enzymes involved in sex steroid metabolism. NO synthase activity was also detected in the villi of a complete mole placenta (which lacks fetal vessels), further supporting a trophoblastic origin. Our findings suggest a previously unrecognized role for STr‐derived NO in placental function.—Conrad, K. P., Villi, M., McGuire, P. G., Dail, W. G., Davis, A. K. Expression of nitric oxide synthase by syncytiotrophoblast in human placental villi.FASEB J.7: 1269‐1276; 1993.