Similarity of antibiotic resistance patterns and molecular typing properties of methicillin-resistant Staphylococcus aureus isolates widely spread in hospitals in New York City and in a hospital in Tokyo, Japan
Similarity of antibiotic resistance patterns and molecular typing properties of methicillin-resistant Staphylococcus aureus isolates widely spread in hospitals in New York City and in a hospital in Tokyo, Japan
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DOI：
10.1089/mdr.2000.6.253
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发表时间：
2000-09-01
期刊：
影响因子：
--
通讯作者：
Tomasz, A
中科院分区：
文献类型：
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作者：
De Sousa, MA;De Lencastre, H;Tomasz, A
1997年4月至6月和1998年2月，在日本东京的一家医院收集了43株单患者耐甲氧西林金黄色葡萄球菌（MRSA），采用分子分型技术对ClaI酶切酶切与mecA和tn554特异性DNA探针杂交，并通过脉冲场凝胶电泳（PFGE）测定smai酶切的染色体DNA的宏观酶切模式进行了鉴定。大部分（76%）分离株携带mecA多态性I、Tn554型A和PFGE型A（克隆型I:A:A），与纽约医院和邻近的新泽西州、康涅狄格州和宾夕法尼亚州医院广泛传播的MRSA克隆型相同。与纽约克隆相似，日本医院的MRSA分离株大部分对青霉素、环丙沙星、红霉素、四环素耐药。对氯霉素、磺胺甲恶唑-甲氧苄啶和利福平敏感，143株MRSA的万古霉素mic均小于或等于2 mg /ml。
One hundred and forty-three single-patient methicillin-resistant Staphylococcus aureus (MRSA) isolates collected during April-June, 1997, and February, 1998, in a hospital in Tokyo, Japan, were characterized by molecular typing techniques that involved hybridization of ClaI restriction digests with the mecA- and Tn554-specific DNA probes and determination of macrorestriction patterns of SmaI-digested chromosomal DNA by pulsed-field gel electrophoresis (PFGE), A large proportion (76%) of the isolates carried the mecA polymorph I, Tn554 pattern A, and PFGE pattern A (clonal type I:A:A), which was the same as the clonal type of an MRSA widely spread in hospitals in New York City and hospitals in neighboring New Jersey, Connecticut, and Pennsylvania, Also similarly to the New York clone, most of the MRSA isolates from the Japanese hospital were resistant to penicillin, ciprofloxacin, erythromycin, tetracycline, and high concentrations (500 mug/ml) of spectinomycin, but were susceptible to chloramphenicol, sulfamethoxazole-trimethoprim, and rifampin, All of the 143 MRSA isolates had vancomycin MICs less than or equal to2 mgL.