Distinguishing angioimmunoblastic T-cell lymphoma from peripheral T-cell lymphoma, unspecified, using morphology, immunophenotype and molecular genetics
Distinguishing angioimmunoblastic T-cell lymphoma from peripheral T-cell lymphoma, unspecified, using morphology, immunophenotype and molecular genetics
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DOI：
10.1111/j.1365-2559.2007.02632.x
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发表时间：
2007-03-01
期刊：
影响因子：
6.4
通讯作者：
Dogan, A.
中科院分区：
文献类型：
--
作者：
Attygalle, A. D.;Chuang, S-S;Dogan, A.
目的：目的探讨血管免疫母细胞性T细胞淋巴瘤（AITL）与外周T细胞淋巴瘤（PTL）的组织学、免疫表型和分子遗传学特征。检查的淋巴结T细胞淋巴瘤（n =137），包括AITL（n = 89），PTL（n = 22），间变性大细胞淋巴瘤（n = 16）和“AITL/PTL不确定”（n = 10），在AITL和PTL之间具有重叠特征，显示典型的AITL形态，但缺乏滤泡树突状细胞扩增。结果：89例AITLs中，74/89例为典型形态，15/89例为增生卵泡。AITL和“AITL/PTL不确定”在所有病例中均显示多形浸润和显著的血管分布。在两组中，CD 10大多数存在，透明细胞和EBER阳性是PTL缺乏的特异性（但不是普遍的）特征。AITL中T细胞克隆性的检测（90%）显著高于PTLu（59%.Conclusions）：透明细胞和EBV感染（当存在时）是AITL的有用的鉴别特征，CD 10是AITL的敏感和特异的标志物。增生性卵泡存在于极少数的AITL中。AITL/PTL不确定可能福尔斯AITL而不是PTL的范围。
Aims: To identify distinguishing histological, immunophenotypic and molecular genetic features between angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphoma (PTL).Methods: Nodal T-cell lymphomas examined (n =137), included AITL (n = 89), PTL (n = 22), anaplastic large cell lymphoma (n = 16) and 'AITL/PTL indeterminate' (n = 10) with overlapping features between AITL and PTL, showing morphology typical of AITL but lacking follicular dendritic cell expansion. Immunohistochemistry for CD3, CD20, CD21 and CD10, in situ hybridization for Epstein-Barr virus encoded RNA (EBER) and polymerase chain reaction for T-cell and B-cell clonality analysis were performed.Results: Of the AITLs, 74/89 showed typical morphology, whereas 15/89 showed hyperplastic follicles. AITL and 'AITL/PTL indeterminate' showed a polymorphous infiltrate and prominent vascularity in all cases. In both groups, CD10 was present in the majority and clear cells and EBER positivity were specific (but not universal) features lacking in PTL. Detection of T-cell clonality was significantly higher in AITL (90%) compared with PTLu (59%).Conclusions: Clear cells and EBV infection (when present) are useful distinguishing features and CD10 a sensitive and specific marker of AITL. Hyperplastic follicles are present in a significant minority of AITL. AITL/PTL indeterminate probably falls within the spectrum of AITL rather than PTL.