Brain neurosteroids during the mouse oestrous cycle
Brain neurosteroids during the mouse oestrous cycle
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DOI：
10.1016/s0006-8993(97)00749-x
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发表时间：
1997-08-22
期刊：
影响因子：
2.9
通讯作者：
Fry, JP
中科院分区：
文献类型：
--
作者：
Corpechot, C;Collins, BE;Fry, JP
在整个发情周期中，测量了小鼠脑中神经活性类固醇3 α，5 α-四氢孕酮（TH PROG或别孕烯醇酮）及其前体孕酮（PROG）和5 α-二氢孕酮（DH PROG）的浓度。还测量了血浆PROG浓度以进行比较。在每个阶段，脑PROG及其代谢产物的浓度昼夜波动。这种波动大于发情周期中任何特定阶段的波动。在整个周期中，脑TH PROG（或DH PROG）和PROG浓度之间存在显著相关性，但脑TH PROG（或DH PROG）和血浆PROG浓度之间不存在显著相关性。在全脑匀浆中，内源性TH PROG（或DH PROG）与将H-3-PROG转化为H-3-DH FROG的5 α-还原酶的活性之间也没有相关性。另一种神经活性类固醇，硫酸双烯醇酮（PREG S），在大脑中的浓度在发情周期与血浆PROG，但不是大脑PROG浓度的阶段。我们的研究结果表明，昼夜节律和卵巢的影响，PROG及其代谢产物TH PROG的浓度在雌性小鼠全脑主要是由组织内的PROG供应的变化，无论周边来源的贡献。(C)1997年Elsevier Science B.V.
Concentrations of the neuroactive steroid 3 alpha,5 alpha-tetrahydroprogesterone (TH PROG or allopregnanolone) and its precursors progesterone (PROG) and 5 alpha-dihydroprogesterone (DH PROG) have been measured in mouse brain throughout the oestrous cycle. Plasma PROG concentrations were also measured for comparison. At each stage, circadian fluctuations were found in the concentrations of brain PROG and its metabolites. Such fluctuations were greater than those attributable to any particular stage of the oestrous cycle. Over the entire cycle, a significant correlation was found between brain TH PROG (or DH PROG) and PROG concentrations but not between brain TH PROG (or DH PROG) and plasma PROG concentrations. There was also no correlation between endogenous TH PROG (or DH PROG) and activity of the 5 alpha-reductase converting H-3-PROG to H-3-DH FROG in whole brain homogenates. Concentrations of another neuroactive steroid, pregnenolone sulphate (PREG S), in the brain during the oestrous cycle were in phase with plasma PROG but not brain PROG concentrations. Our results indicate that circadian and ovarian influences on the concentrations of PROG and its metabolite TH PROG in female whole mouse brain are caused predominantly by changes in the supply of PROG from within the tissue, whatever the contribution of peripheral sources. (C) 1997 Elsevier Science B.V.