Isolation of a parvovirus from dogs in Britain
Isolation of a parvovirus from dogs in Britain
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英国从狗身上分离出细小病毒
DOI：
10.1136/vr.105.8.167
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发表时间：
1979
影响因子：
2.2
通讯作者：
N. Wright
中科院分区：
文献类型：
--
作者：
I. McCandlish;H. Thompson;H. Cornwell;H. Laird;N. Wright
通过电子显微镜在突然出现呕吐和大量水样灰色粪便（有时带血斑）的年轻犬的粪便和肠道物质中鉴定出细小病毒。将病毒培养于犬肾细胞系（GH）和猫胚胎细胞系（FEA）中；后者繁殖效果最好。在一例心肌炎中也发现细小病毒样颗粒。3-4周龄急性致死性心肌炎幼犬无肠道疾病史，8-10周龄肠炎成年犬和幼犬无心脏病变。然而，一些5-6周龄的小狗，主要表现为肠炎，在心肌细胞中有核内包涵体，尽管没有出现活动性心肌炎。不同的心脏和肠道综合征可能反映了细小病毒对快速生长和分裂细胞的倾向，以及不同年龄心脏和肠道细胞生长速度的差异。
A parvovirus was identified by electron microscopy in faecal and intestinal material from young dogs with sudden onset of vomiting and the passing of profuse, watery greyish faeces sometimes flecked with blood. The virus was cultured in a continuous dog kidney cell line (GH) and a feline embryonic cell line (FEA); propagation was best in the latter. Parvovirus-like particles were also identified in one case of myocarditis. Young puppies 3-4 weeks old with acute fatal myocarditis had had no history of intestinal disease, while adult dogs and puppies of 8-10 weeks old with enteritis did not show cardiac lesions. However, some puppies aged 5-6 weeks and primarily showing enteritis had intranuclear inclusions in cardiac myocytes, although no active myocarditis was present. The different cardiac and enteric syndrome may reflect the tropism of parvoviruses for rapidly growing and dividing cells and the differences in the growth rates of cardiac and intestinal cells at different ages.