Expression of the insulin-like growth factors and their receptors in adenocarcinoma of the colon
Expression of the insulin-like growth factors and their receptors in adenocarcinoma of the colon
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作者：
Freier, S;Weiss, O;Raz, I
目的-研究胰岛素样生长因子（IGFs）及其受体的表达变化，以及TGF-Ⅰ和IGF-II多肽的产生，在结肠腺癌。用RNA酶保护法检测IGF-Ⅰ、IGF-Ⅱ及其受体mRNA的表达。IGF-I和IGF-II多肽测定特异性immunoassays.Results-All正常组织表达IGF-II，IGF-I受体，IGF-II/甘露糖-6-磷酸（Man-6-P）受体。IGF-I mRNA不能被检测到，但多肽存在于正常和恶性组织中的少量但相等的量。IGF-II在结肠肿瘤中的表达是邻近正常组织的40倍，相应多肽的浓度是恶性组织的两倍。IGF-I受体表达增加2.5倍，IGF-II/Man-6-P受体增加4倍。结论：本研究证实，人结肠癌中IGF-I受体和IGF-II的表达增加。此外，IGF-II/Man-6-P受体信息增加，并且IGF-II信息的增加伴随着肿瘤组织中IGF-IE蛋白与邻近正常组织相比加倍。这些发现表明。IGF-II/Man-6-P受体也可能参与结肠腺癌的发展。有迅速积累的证据表明，IGF系统在大肠恶性肿瘤的发展。
Aims-To study changes in the expression of insulin-like growth factors (IGFs) and their receptors, as well as production of the TGF-I and IGF-II polypeptides, in adenocarcinoma of the colon.Methods-Malignant tissue obtained at operation was used. Total RNA was at acted and specific IGF-I and IGF-II and their receptor mRNAs were measured by a solution hybridisation RNase protection assay. IGF-I and IGF-II polypeptides were measured by specific immunoassays.Results-All normal tissues expressed IGF-II, IGF-I receptor, and IGF-II/mannose-6-phosphate (Man-6-P) receptor. IGF-I mRNA could not be detected but the polypeptide was present in small but equal amounts in normal and malignant tissue. IGF-II was expressed 40 times more abundantly in colonic tumours than in adjacent normal tissue and the concentration of the corresponding polypeptide was twice as high in the malignant tissue. IGF-I receptor expression was increased by a factor of 2.5 and IGF-II/Man-6-P receptor by a factor of 4.Conclusions-This study confirms that ire adenocarcinoma of the human colon there is increased expression of IGF-I receptor and IGF-II. Furthermore, IGF-II/Man-6-P receptor message is increased and the increase in IGF-II message is accompanied by a doubling of the IGF-IE protein in the tumour tissue compared with, the adjacent normal tissue. These findings suggest that. the IGF-II/Man-6-P receptor may also be involved in development of adenocarcinoma of the colon. There is rapidly accumulating evidence implicating the IGF system in the development of malignancy of the large bowel.