Olfactory sensory neurons expressing class I odorant receptors converge their axons on an antero-dorsal domain of the olfactory bulb in the mouse
Olfactory sensory neurons expressing class I odorant receptors converge their axons on an antero-dorsal domain of the olfactory bulb in the mouse
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DOI：
10.1111/j.1460-9568.2006.04675.x
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发表时间：
2006-03-01
影响因子：
3.4
通讯作者：
Sakano, H
中科院分区：
文献类型：
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作者：
Tsuboi, A;Miyazaki, T;Sakano, H
脊椎动物气味受体基因在遗传学上分为两类：类鱼类的I类和陆地特有的II类。在本研究中，我们系统地分析了小鼠I类OR基因（42个亚家族），以阐明在嗅上皮（OE）的表达谱及其嗅感觉神经元（OSNs）在嗅球（OB）的投射部位。原位杂交（ISH）显示，大多数I类OR基因（36个亚家族）表达的背内侧区（1区）的OE。此外，似乎没有显着差异的OSNs表达I类基因的分布在1区。这些结果表明，有一个明确的边界之间的1区和非1区的OE。一些I类OR是已知的具有配体特异性的脂肪酸，醛和醇。我们的ISH分析表明，在1区中表达I类OR的OSN倾向于将其轴突会聚在前背域中的肾小球簇上，该前背域被认为参与对OB上的脂肪族化合物的响应。
Vertebrate odorant receptor (OR) genes are divided phylogenetically into two distinct classes: the fish-like class I and the terrestrial-specific class II. In the present study, we systematically analysed mouse class I OR genes (42 subfamilies) to elucidate the expression profiles in the olfactory epithelium (OE) and the projection sites of their olfactory sensory neurons (OSNs) in the olfactory bulb (OB). In situ hybridization (ISH) revealed that most class I OR genes (36 subfamilies) were expressed in the dorso-medial zone (zone 1) of the OE. Furthermore, there appeared to be no significant differences in the distributions of OSNs expressing class I genes within zone 1. These results indicate that there is a clear boundary between zone 1 and non-zone 1 areas in the OE. Some class I ORs are known to possess ligand specificity for aliphatic acids, aldehydes and alcohols. Our ISH analysis has revealed that OSNs expressing the class I ORs in zone 1 tend to converge their axons on a cluster of glomeruli in an antero-dorsal domain that is assumed to be involved in responses to the aliphatic compounds on the OB.