Development of novel electrospun absorbable polycaprolactone (PCL) scaffolds for hernia repair applications
Development of novel electrospun absorbable polycaprolactone (PCL) scaffolds for hernia repair applications
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DOI：
10.1007/s00464-012-2258-8
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发表时间：
2012-10-01
影响因子：
3.1
通讯作者：
Deeken, Corey R.
中科院分区：
文献类型：
--
作者：
Ebersole, Gregory C.;Buettmann, Evan G.;Deeken, Corey R.
永久性/不可吸收的疝气修复材料依赖于促纤维化伤口愈合，修复部位通常由混乱的组织组成，机械强度较差，存在再次疝出的风险。可吸收性静电纺丝支架是一类新型的生物材料，为设计先进的软组织修复材料提供了一个独特的平台。这些材料是简单、廉价的非织造材料，由容易模仿天然细胞外基质的聚合物纤维组成。本研究的主要目的是评估新型静电纺丝支架的物理力学性能，以确定其适用于疝修补术。根据以往的实验结果，具有千分之一日元20 N缝线保留强度，千分之一日元20 N撕裂强度和千分之一日元50 N/cm拉伸强度的支架适合于疝修补。简言之，将聚（ε-己内酯）（PCL）溶解在溶剂混合物中并静电纺丝到平面金属收集器上，产生具有随机取向纤维的片材。扫描电子显微镜照片显示纤维直径范围从1.0 +/-A0.1 μ m（10%PCL，3.5 mL/h）到1.5 +/-A0.2 μ m（12%PCL，4 mL/h）。激光测微仪显示厚度范围为0.72 +/- A 0.07 mm（12% PCL，10 mL/h）至0.91 +/- A 0.05 mm（10% PCL，3.5 mL/h）。机械测试确定了两种具有缝线保持强度的支架（12%PCL，10 mL/h和12%PCL，6 mL/h），并且没有支架具有撕裂强度值的支架（范围，4.7 +/- A 0.9 N至10.6 +/- A 1.8 N）。拉伸强度范围为35.27 +/- A 2.08 N/cm（10% PCL，3.5 mL/h）至81.76 +/- A 15.85 N/cm（12%PCL，4 mL/h），三种支架的强度为千分之一日元50 N/cm两种电纺支架（12%PCL，10 mL/h和12%PCL，6 mL/h）具有适合于疝修补的缝合线保留和拉伸强度，证明了在大型动物模型中的评价是合理的。进一步研究先进的制造方法可能会进一步改善这些支架的独特性能，推动它们在各种临床环境中的应用。
Permanent/nonresorbable hernia repair materials rely on profibrotic wound healing, and repair sites are commonly composed of disorganized tissue with inferior mechanical strength and risk of reherniation. Resorbable electrospun scaffolds represent a novel class of biomaterials, which may provide a unique platform for the design of advanced soft tissue repair materials. These materials are simple, inexpensive, nonwoven materials composed of polymer fibers that readily mimic the natural extracellular matrix. The primary goal of the present study was to evaluate the physiomechanical properties of novel electrospun scaffolds to determine their suitability for hernia repair. Based on previous experimentation, scaffolds possessing a parts per thousand yen20 N suture retention strength, a parts per thousand yen20 N tear resistance, and a parts per thousand yen50 N/cm tensile strength are appropriate for hernia repair.Six novel electrospun scaffolds were fabricated by varying combinations of polymer concentration (10-12 %) and flow rate (3.5-10 mL/h). Briefly, poly(epsilon-caprolactone) (PCL) was dissolved in a solvent mixture and electrospun onto a planar metal collector, yielding sheets with randomly oriented fibers. Physiomechanical properties were evaluated through scanning electron microscopy, laser micrometry, and mechanical testing.Scanning electron micrographs demonstrated fiber diameters ranging from 1.0 +/- A 0.1 mu m (10 % PCL, 3.5 mL/h) to 1.5 +/- A 0.2 mu m (12 % PCL, 4 mL/h). Laser micrometry demonstrated thicknesses ranging from 0.72 +/- A 0.07 mm (12 % PCL, 10 mL/h) to 0.91 +/- A 0.05 mm (10 % PCL, 3.5 mL/h). Mechanical testing identified two scaffolds possessing suture retention strengths a parts per thousand yen20 N (12 % PCL, 10 mL/h and 12 % PCL, 6 mL/h), and no scaffolds possessing tear resistance values a parts per thousand yen20 N (range, 4.7 +/- A 0.9 N to 10.6 +/- A 1.8 N). Tensile strengths ranged from 35.27 +/- A 2.08 N/cm (10 % PCL, 3.5 mL/h) to 81.76 +/- A 15.85 N/cm (12 % PCL, 4 mL/h), with three scaffolds possessing strengths a parts per thousand yen50 N/cm (12 % PCL, 10 mL/h; 12 % PCL, 6 mL/h; 12 % PCL, 4 mL/h).Two electrospun scaffolds (12 % PCL, 10 mL/h and 12 % PCL, 6 mL/h) possessed suture retention and tensile strengths appropriate for hernia repair, justifying evaluation in a large animal model. Additional studies examining advanced methods of fabrication may further improve the unique properties of these scaffolds, propelling them into applications in a variety of clinical settings.