Distribution of particulate matter and tissue remodeling in the human lung
Distribution of particulate matter and tissue remodeling in the human lung
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DOI：
10.2307/3434960
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发表时间：
2000-11-01
影响因子：
10.4
通讯作者：
Schenker, MB
中科院分区：
文献类型：
--
作者：
Pinkerton, KE;Green, FHY;Schenker, MB
我们研究了来自加州中央山谷的西班牙裔男性肺内碳质粉尘和矿物粉尘的颗粒分布与沿着解剖上不同的呼吸道路径的呼吸道重塑之间的关系。肺尸检标本取自弗雷斯诺县验尸官办公室，用2%戊二醛在30厘米水压力下气管内滴注制备。沿着两条不同的气道路径进入左上肺叶的心尖后部和心尖前部。组织学分析的组织样本通常取自肺内第二、第四、第六和第九代气道。并对各通道第12代以上的实质组织进行分析。除了与支气管相关的淋巴组织和支气管周围结缔组织外，在较大的传导气道(第2-6代)中几乎没有可见颗粒聚集的证据。相反，来自每个通路的终末和呼吸性细支气管显示不同程度的管壁增厚和重塑。有明显增厚的墙壁含有中到大量的碳质和矿物粉尘。管壁增厚与胶原和间质炎症细胞的增加有关，包括尘埃缠绕的巨噬细胞。与第二代和第三代呼吸性细支气管组相比，第一代呼吸性细支气管组的这些变化明显更大。这些发现表明，碳质粉尘和矿物粉尘在肺部的积聚受肺解剖结构的显著影响，肺腺泡中心的滞留最多。此外，暴露在周围颗粒物中的人的这一过渡区发生了显著的重塑。
We examined the relationship between intrapulmonary particle distribution of carbonaceous and mineral dusts and remodeling of the airways along anatomically distinct airway paths in the lungs of Hispanic males from the central valley of California. Lung autopsy specimens from the Fresno County Coroner's Office were prepared by intratracheal instillation of 2% glutaraldehyde at 30 cm H2O pressure. Two distinct airway paths into the apico-posterior and apico-anterior portions of the left upper lung lobe were followed. Tissue samples for histologic analysis were generally taken from the intrapulmonary second, fourth, sixth, and ninth airway generations. Parenchymal tissues beyond the 12th airway generation of each airway path were also analyzed. There was little evidence of visible particle accumulation in the larger conducting airways (generations 2-6), except in bronchial-associated lymphoid tissues and within peribronchial connective tissue. In contrast, terminal and respiratory bronchioles arising from each pathway revealed varying degrees of wall thickening and remodeling. Walls with marked thickening contained moderate to heavy amounts of carbonaceous and mineral dusts. Wall thickening was associated with increases in collagen and interstitial inflammatory cells, including dust-laden macrophages. These changes were significantly greater in first-generation respiratory bronchioles compared to second- and third-generation respiratory bronchioles. These findings suggest that accumulation of carbonaceous and mineral dust in the lungs is significantly affected by lung anatomy with the greatest retention in centers of lung acini. Furthermore, there is significant remodeling of this transitional zone in humans exposed to ambient particulate matter.