Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery
Distribution of adrenomedullin-containing perivascular nerves in the rat mesenteric artery
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DOI：
10.1016/j.peptides.2004.02.005
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发表时间：
2004-04-01
期刊：
影响因子：
3
通讯作者：
Kawasaki, H
中科院分区：
文献类型：
--
作者：
Hobara, N;Nakamura, A;Kawasaki, H
本文研究了含肾上腺髓质素（AM）的血管周围神经纤维在大鼠肠系膜动脉的分布。外膜中观察到许多含有AM样免疫反应性（LI）的纤维。AM-LI纤维被取消冷存储去神经或辣椒素，但不是6-羟基多巴胺。免疫双重染色显示AM-LI与降钙素基因相关肽（CGRP）-LI共定位。RT-PCR检测到背根神经节内有大量AM阳性细胞和AM mRNA表达。电镜下可见高比例的AM免疫金标记，AM-LI和CGRP-LI共定位于无髓神经轴突。这些结果表明，含AM的血管周围神经分布在大鼠肠系膜动脉。(C)2004年爱思唯尔公司All rights reserved.
Distribution of adrenomedullin (AM)-containing perivascular nerve fibers was studied in rat mesenteric arteries. Many fibers containing AM-like immunoreactivity (LI) were observed in the adventitia. AM-LI fibers were abolished by cold storage denervation or capsaicin but not 6-hydroxydopamine. Double immunostainings showed colocalization of AM-LI with calcitonin gene-related peptide (CGRP)-LI. The dorsal root ganglia had many AM-positive cells and AM mRNA detected by RT-PCR. Electron microscopy study revealed high proportions of immunogold labeling for AM and colocalization of both AM-LI and CGRP-LI in unmyelinated nerve axons. These results suggest that AM-containing perivascular nerves are distributed in the rat mesenteric artery. (C) 2004 Elsevier Inc. All rights reserved.