Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors
Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors
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DOI：
10.1073/pnas.1110854108
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发表时间：
2011-09-13
影响因子：
11.1
通讯作者：
Cepko, Constance L.
中科院分区：
文献类型：
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作者：
Beier, Kevin T.;Saunders, Arpiar;Cepko, Constance L.
要了解神经系统是如何处理信息的，神经元之间的连接图将大有裨益。在这里，我们描述了使用水疱性口炎病毒（VSV）在体内追踪神经元连接。我们利用其他几种病毒的糖蛋白(G)基因制作了VSV载体。来自淋巴细胞性脉络丛脑膜炎病毒的G蛋白赋予VSV跨突触传播的能力，特别是在顺行方向上，而狂犬病毒糖蛋白则具有特异性的逆行跨突触模式。使用禽G蛋白融合可以特异性靶向表达禽受体的细胞，从而证明了从确定的细胞进行单突触顺行追踪。利用切片培养和电生理学证实了病毒标记细胞对的突触连通性。小鼠大脑中几个区域的体内感染导致了顺行或逆行示踪剂的预测传播模式。
To understand how the nervous system processes information, a map of the connections among neurons would be of great benefit. Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo. We made VSV vectors that used glycoprotein (G) genes from several other viruses. The G protein from lymphocytic choriomeningitis virus endowed VSV with the ability to spread transsynaptically, specifically in an anterograde direction, whereas the rabies virus glycoprotein gave a specifically retrograde transsynaptic pattern. The use of an avian G protein fusion allowed specific targeting of cells expressing an avian receptor, which allowed a demonstration of monosynaptic anterograde tracing from defined cells. Synaptic connectivity of pairs of virally labeled cells was demonstrated by using slice cultures and electrophysiology. In vivo infections of several areas in the mouse brain led to the predicted patterns of spread for anterograde or retrograde tracers.