Chromosome identification in Cucumis anguria revealed by cross-species single-copy gene FISH
Chromosome identification in Cucumis anguria revealed by cross-species single-copy gene FISH
复制标题
跨物种单拷贝基因FISH揭示黄瓜染色体鉴定
DOI：
10.1139/gen-2017-0235
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发表时间：
2018-06-01
期刊：
影响因子：
3.1
通讯作者：
Lou, Qunfeng
中科院分区：
文献类型：
--
作者：
Li, Ziang;Bi, Yunfei;Lou, Qunfeng
黄瓜具有广谱抗病性，是黄瓜属作物改良的潜在遗传资源。然而，对C.由于其小的中期染色体和缺乏可区分的染色体界标，因此已有anguria的报道。本研究以黄瓜14个单拷贝基因和rDNA为探针，对黄瓜染色体进行FISH分析。安古里亚。探针的独特信号分布模式使我们能够区分C.无尿症（A01-A12）。进一步通过粗线期染色体FISH获得了详细的染色体特征。粗线期染色体长度为54.80 ~ 143.41 μ m。异染色质区的比例从13.56%到63.86%不等。最后，探讨了C.黄瓜（C1-C7）。结果表明，A06 + A09、A03 + A12、A02 + A04和A01 + A11分别是C1、C2、C3和C6的同源物。A08、A10和A05分别与C4、C5和C7同源。染色体鉴定及亲缘关系分析。anguria和黄瓜基因组结构的研究为进一步研究黄瓜属物种的基因组结构进化奠定了基础。
Cucumis anguria is a potential genetic resource for improving crops of the genus Cucumis, owing to its broad-spectrum resistance. However, few cytogenetic studies on C. anguria have been reported because of its small metaphase chromosomes and the scarcity of distinguished chromosomal landmarks. In this study, 14 single-copy genes from cucumber and rDNAs were used as probes for FISH to identify the individual chromosomes of C. anguria. The distinctive signal distribution patterns of the probes allowed us to distinguish each chromosome of C. anguria (A01-A12). Further, detailed chromosome characteristics were obtained through pachytene chromosome FISH. The lengths of pachytene chromosomes varied from 54.80 to 143.41 mu m. The proportion of heterochromatin regions varied from 13.56% to 63.86%. Finally, the chromosomal homeologous relationship between C. anguria and cucumber (C1-C7) was analyzed. The results showed that A06 + A09, A03 + A12, A02 + A04, and A01 + A11 were homeologs of C1, C2, C3, and C6, respectively. Furthemore, chromosomes A08, A10, and A05 were homeologs of C4, C5, and C7, respectively. Chromosome identification and homeologous relationship analysis between C. anguria and cucumber lay the foundation for further research of genome structure evolution in species of Cucumis.