Expressions of Matrix Metalloproteinases 2, 7, and 9 in Carcinogenesis of Pancreatic Ductal Adenocarcinoma.
Expressions of Matrix Metalloproteinases 2, 7, and 9 in Carcinogenesis of Pancreatic Ductal Adenocarcinoma.
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DOI：
10.1155/2016/9895721
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Guzińska-Ustymowicz K
中科院分区：
文献类型：
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作者：
Jakubowska K;Pryczynicz A;Januszewska J;Sidorkiewicz I;Kemona A;Niewiński A;Lewczuk Ł;Kędra B;Guzińska-Ustymowicz K
胰腺导管腺癌（PDAC）是一种高度致命的疾病，通常在晚期被诊断出来，只有很小的康复机会。基质金属蛋白酶（matrix metalloproteinases，MMPs）是一类锌依赖性内肽酶，参与组织重塑，刺激新生血管形成和炎症反应。本研究的目的是评估MMP-2、MMP-7和MMP-9在正常导管、肿瘤胰腺癌细胞和瘤周间质中的表达与临床组织病理学参数的相关性。研究材料来自29例胰腺导管腺癌患者。免疫组化法检测MMP-2、MMP-7、MMP-9的表达。微血管密度（MVD）通过特殊免疫染色显示。MMP-2、MMP-7、MMP-9主要表达于肿瘤细胞及瘤周间质。MMP-2在癌细胞中的表达与女性性别、较强的炎症和癌症的组织病理学类型相关（分别为R = 0.460，p = 0.013; R = 0.690，p = 0.0001; R =-0.440，p = 0.005）。MMP-7在肿瘤细胞中的表达与坏死呈正相关，与MVD呈负相关（R = 0.402，p = 0.031; R =-0.682，p = 0.000）。MMP-9阳性表达与MVD相关（R = 0.368，p = 0.084），但无统计学意义。我们的研究结果表明，MMP-2，MMP-7和MMP-9的表达与PDAC肿瘤的各种形态学特征，如炎症，坏死和新血管的形成相关。
Pancreatic ductal adenocarcinoma (PDAC) is a highly fatal disease, usually diagnosed in an advanced stage which gives a slight chance of recovery. Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that participate in tissue remodeling and stimulate neovascularization and inflammatory response. The aim of the study was to evaluate the expression of MMP-2, MMP-7, and MMP-9 in normal ducts, tumor pancreatic adenocarcinoma cells, and peritumoral stroma in correlation with clinicohistopathological parameters. The study material was obtained from 29 patients with pancreatic ductal adenocarcinoma. The expressions of MMP-2, MMP-7, and MMP-9 were performed by immunohistochemical technique. Microvessel density (MVD) was visualized by special immunostaining. The expressions of MMP-2, MMP-7, and MMP-9 were mainly observed in tumor cells and peritumoral stroma. MMP-2 expression in cancer cells was correlated with female gender, stronger inflammation, and histopathological type of cancer (R = 0.460, p = 0.013; R = 0.690, p = 0.0001; R = −0.440, p = 0.005, resp.). The expression of MMP-7 in tumor cells was found to positively correlate with the presence of necrosis and negatively correlate with MVD (R = 0.402, p = 0.031; R = −0.682, p = 0.000). We also showed that positive MMP-9 expression in tumor cells was associated with MVD (R = 0.368, p = 0.084); however, it was not statistically significant. Our results demonstrate that MMP-2, MMP-7, and MMP-9 expressions correlate with various morphological features of the PDAC tumor such as inflammation, necrosis, and formation of the new blood vessels.