Chemical Protein Synthesis by Chemoselective #x03B1;-Ketoacid?Hydroxylamine (KAHA) Ligations with 5-Oxaproline
Chemical Protein Synthesis by Chemoselective #x03B1;-Ketoacid?Hydroxylamine (KAHA) Ligations with 5-Oxaproline
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化学选择性化学蛋白质合成
DOI：
10.1007/978-1-0716-1617-8_14
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Bode Jeffrey W.
中科院分区：
文献类型：
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作者：
Farnung Jakob;Song Haewon;Bode Jeffrey W.
化学蛋白质合成通过采用固相肽合成和化学选择性连接来实现蛋白质的精确构建。一种这样的适合于蛋白质合成的化学选择性反应是α-酮酸-羟胺（KAHA）连接。完全未保护的肽通过α-酮酸和羟胺之间的选择性反应连接，产生天然酰胺键。在这里，我们描述了泛素的化学合成的两段方法使用5-氧脯氨酸羟胺。
Chemical protein synthesis enables the precise construction of proteins by employing solid-phase peptide synthesis and chemoselective ligations. One such chemoselective reaction suitable for protein synthesis is the α-Ketoacid-Hydroxylamine (KAHA) ligation. Fully unprotected peptides are ligated by a selective reaction between α-ketoacids and hydroxylamines to give native amide bonds. Herein, we describe the chemical synthesis of ubiquitin by a two-segment approach using the 5-oxaproline hydroxylamine.