Gene silencing using a heat-inducible RNAi system in Arabidopsis
Gene silencing using a heat-inducible RNAi system in Arabidopsis
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DOI：
10.1016/j.bbrc.2004.06.154
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发表时间：
2004-08-20
影响因子：
3.1
通讯作者：
Galaud, JP
中科院分区：
文献类型：
--
作者：
Masclaux, F;Charpenteau, M;Galaud, JP
调控植物发育过程中基因的表达是研究基因功能的有效工具。在本文中，我们描述了一个由热休克基因启动子（HSP 18.2）驱动的基因表达系统，以触发含有内含子的反向重复序列的表达。RNA干扰技术已成为反向遗传学研究基因功能的有力手段。然而，组成型基因沉默不能用于参与基本过程如胚胎活力的基因。诱导型启动子为时空基因表达控制提供了另一种方法，我们在这里描述了一种新的系统，与使用化学基因诱导剂的系统互补。为了评估该系统的效率，将对应于拟南芥八氢番茄红素去饱和酶基因的RNA用作转基因植物中的报告基因，并使用CaMV 35S组成型启动子或HSP 18.2诱导型启动子进行比较研究。(C)2004年爱思唯尔公司All rights reserved.
Controlling gene expression during plant development is an efficient tool to explore gene function. In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing inverted-repeat. RNA interference became a powerful way for gene functional analysis by reverse genetic approaches. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability. Inducible promoters provide an alternative approach for temporal and spatial gene expression control and we described here a new system, complementary to those using chemical gene inducers. To evaluate the efficiency of this system, RNA corresponding to the phytoene desaturase gene of Arabidopsis thaliana was used as a reporter gene in transgenic plants and a comparative study was performed using either the CaMV35S constitutive promoter or the HSP18.2 inducible promoter. (C) 2004 Elsevier Inc. All rights reserved.