DISTRIBUTION OF CRF-LIKE IMMUNOREACTIVITY IN THE RABBIT
DISTRIBUTION OF CRF-LIKE IMMUNOREACTIVITY IN THE RABBIT
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DOI：
10.1016/0196-9781(82)90025-0
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发表时间：
1982-01-01
期刊：
影响因子：
3
通讯作者：
MOLDOW, RL
中科院分区：
文献类型：
--
作者：
FISCHMAN, AJ;MOLDOW, RL
用放射免疫法测定正常家兔脑和胃肠胰管的crf样免疫反应性。在大脑中检测到它，在下丘脑腹侧浓度最高。免疫反应性在兔脑中的分布比在大鼠脑中的分布要有限得多，大量的肽仅在靠近下丘脑的区域检测到，如丘脑、视前区、中脑和杏仁核。在Sephadex G-50层析上，相对于大鼠CRF样免疫反应性和合成羊CRF，下丘脑外免疫反应性略有延迟。肺、胰腺、十二指肠、胃窦沸水提取物未见明显的crf样免疫反应性。这些数据与先前关于Sephadex G-50仅在下丘脑中的空洞体积免疫反应性的报道相结合，表明CRF仅在下丘脑中合成，而不是在整个胃肠道和中枢神经系统中发现的肽类成员。
CRF-like immunoreactivity was measured by radioimmunoassay in the brains and gastroenteropancreatic tract of normal rabbits. It was detected in the brain with the highest concentration in the ventral hypothalamus. The distribution of immunoreactivity was much more limited in the rabbit brain than in the rat brain with substantial amounts of peptide detected only in areas of close proximity to the hypothalamus e.g., thalamus, preoptic area, midbrain and amygdala. The extrahypothalamus immunoreactivity was slighly retarded on Sephadex G-50 chromatography relative to rat CRF-like immunoreactivity and synthetic ovine CRF. No apparent CRF-like immunoreactivity was detected in boiling water extracts of lung, pancreas, duodenum or antrum. These data in conjunction with a previous report of void volume immunoreactivity on Sephadex G-50 only in the hypothalamus suggest that CRF is synthesized only in the hypothalamus and is not a member of the class of peptides found throughout the gastroenteropancreatic tract and the CNS.