Calcification as an indicator of osteoinductive capacity of biomaterials in osteoblastic cell cultures
Calcification as an indicator of osteoinductive capacity of biomaterials in osteoblastic cell cultures
复制标题
DOI：
10.1016/j.biomaterials.2005.01.025
复制
发表时间：
2005-08-01
期刊：
影响因子：
14
通讯作者：
Cornelissen, MJ
中科院分区：
文献类型：
--
作者：
Declerq, HA;Verbeeck, RMH;Cornelissen, MJ
矿化的细胞外基质形成是生物材料的骨诱导能力的代表，并且经常在体外进行测试。通过von Kossa染色、FTIR、X-射线衍射、TEM和早期（ALP和胶原I形成）和晚期（骨钙素分泌）成骨细胞表达的相关参数，比较了原代大鼠成骨细胞（骨髓、颅骨、骨膜、胎儿和成人长骨）和UMR-106细胞体外矿化的特征。所有培养物均表达高碱性磷酸酶活性，并能够形成骨磷灰石。然而，观察到结节性与弥漫性矿化模式。骨髓、颅骨和骨膜（早期传代）来源的细胞在结节的三维区域上限制性矿化。细胞外基质由I型胶原纤维组成，基质囊泡中载有针状晶体。长骨、晚期传代骨膜来源和UMR-106细胞表现出弥漫性矿化模式。细胞间可见针状结晶，未见胶原纤维和基质囊泡。在源自骨髓的培养物中检测到骨钙素的分泌，而在UMR-106和长骨来源的细胞培养物中不存在。目前的研究表明，营养不良性钙化不能区分与细胞介导的钙化与von Kossa，FTIR和X射线衍射。原代成骨细胞能够形成结节的建议，以评估生物材料的骨诱导性能。(c)2005爱思唯尔有限公司保留所有权利。
Mineralized extracellular matrix formation is representative for the osteoinductive capacity of biomaterials and is often tested in vitro. Characteristics of in vitro mineralization of primary rat osteoblastic cells (bone marrow, calvaria, periosteum, fetal and adult long bone) and UMR-106 cells were compared by von Kossa staining, FTIR, X-ray diffractometry, TEM and related to parameters of early (ALP and collagen I formation) and late (osteocalcin secretion) osteoblast expression. All Cultures expressed high alkaline phosphatase activity and were able to form bone apatite. However, a nodular versus diffuse mineralization pattern was observed. Bone marrow, calvaria and periosteum (early passage) derived cells mineralized restrictively on the three-dimensional area of a nodule. The extracellular matrix consisted of collagen I fibers, among matrix vesicles loaded with needle-like crystals. Long bone, late passage periosteum derived and UMR-106 cells exhibited a diffuse mineralization pattern. Needle-like crystals were observed between the cells but collagen fibers and matrix Vesicles Could not be detected. Secretion of osteocalcin was detected in cultures derived from bone marrow and absent in UMR-106 and long bone derived cell cultures. The present study demonstrates that dystrophic calcification can not be distinguished from cell-mediated calcification with von Kossa, FTIR and X-ray diffractometry. Primary osteoblastic cells capable of forming nodules are recommended to evaluate the osteoinductive properties of biomaterials. (c) 2005 Elsevier Ltd. All rights reserved.