Insight into the regulation of the Nrf2 pathway in response to ascovirus infection in Spodoptera exigua
Insight into the regulation of the Nrf2 pathway in response to ascovirus infection in Spodoptera exigua
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深入研究 Nrf2 通路响应甜菜夜蛾子囊病毒感染的调节
DOI：
10.1002/ps.7284
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发表时间：
2022
影响因子：
4.1
通讯作者：
Guo-Hua Huang
中科院分区：
文献类型：
--
作者：
Ruoheng Jin;Zhengkun Xiao;Madoka Nakai;Guo-Hua Huang
子囊病毒是一类具有较高生物防治潜力的昆虫病原微生物，有望在鳞翅目害虫的自然防治中发挥重要作用。然而，对ascov对其宿主昆虫的生物杀灭活性的分子机制的了解仍然有限。结果在感染HvAV-3h后6 h，超氧化物歧化酶和过氧化物酶的相对活性以及夜蛾过氧化物酶（SePOD）的表达量显著升高。H2O2积累，NADPH氧化酶（SeNOX）表达增强。此外，感染HvAV-3h后，核因子红系2相关因子2 （SeNrf2）和肌腱膜纤维瘤病（SeMaf）过表达。沉默SeNrf2降低了SePOD的表达，而沉默SeNrf2的幼虫死亡率和病毒基因组拷贝数也增加。进一步对SeNOX进行RNA干扰，可显著降低SeNrf2和SePOD的表达，从而增加ascovirus感染宿主的死亡率和病毒基因组拷贝数。结论HvAV-3h激活的葡萄球菌Nrf2/ARE通路和活性氧通过调控Nrf2/ARE通路介导的抗氧化酶基因的改变来应对子囊病毒感染。这些发现增强了我们对子囊病毒与宿主相互作用的认识，为子囊病毒在害虫生物防治中的应用奠定了基础。(c) 2022年化学工业学会。
BACKGROUNDAscoviruses are a type of entomopathogenic microorganism with high biological pest control potential and are expected to contribute to the natural control of lepidopteran pests. However, knowledge of the molecular mechanism underlying the biocidal activity of ascovirus on its host insects remains limited. RESULTSIn this study, the relative enzyme activity of superoxide dismutase and peroxidase, as well as the expression level of Spodoptera exigua peroxidase (SePOD), were found to be significantly increased at 6 h post infection with Heliothis virescens ascovirus 3h (HvAV-3h). H2O2 accumulation and enhanced expression of NADPH Oxidase (SeNOX) were also observed. In addition, Nuclear Factor erythroid 2-Related Factor 2 (SeNrf2) and muscle aponeurosis fibromatosis (SeMaf) were overexpressed following infection with HvAV-3h. Silencing of SeNrf2 decreased the expression of SePOD, whereas the mortality of SeNrf2-silenced larvae and viral genome copy number also increased. Further RNA interference of SeNOX significantly decreased expression of SeNrf2 and SePOD and therefore increased the mortality and viral genome copy number of the ascovirus-infected host. CONCLUSIONThe HvAV-3h activated Nrf2/ARE pathway of S. exigua and reactive oxygen species were found to respond to ascovirus infection by regulating alterations in antioxidant enzyme genes mediated by the host Nrf2/ARE pathway. These findings enhance our knowledge of ascovirus-host interactions and lay the foundation for the application of ascoviruses in biological pest control. (c) 2022 Society of Chemical Industry.