Primary culture of capillary endothelium from rat brain.
Primary culture of capillary endothelium from rat brain.
复制标题
大鼠脑毛细血管内皮的原代培养。
DOI：
10.1007/bf02618147
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Goldstein,GW
中科院分区：
文献类型：
--
作者：
Bowman,PD;Betz,AL;Ar,D;Wolinsky,JS;Penney,JB;Shivers,RR;Goldstein,GW
为了提供用于研究脑毛细血管功能的体外系统，我们开发了一种培养脑毛细血管内皮细胞的方法。从大鼠脑中分离毛细血管并进行酶处理以去除基底膜和污染的周细胞。随后的 Percoll 梯度离心产生了均质的毛细血管内皮细胞群，这些细胞附着在胶原基质上并掺入[3H]胸苷。因子 VIII 抗原和血管紧张素转换酶活性的存在以及血小板未能粘附到细胞表面提供了这些细胞的内皮性质的证据。此外，细胞通过紧密连接连接在一起。因此，这些细胞的原代培养物保留了内皮和血脑屏障特征。
To provide an in vitro system for studies of brain capillary function we developed a method for culture of brain capillary endothelial cells. Capillaries were isolated from rat brain and enzymatically treated to remove the basement membrane and contaminating pericytes. Subsequent Percoll gradient centrifugation resulted in a homogeneous population of capillary endothelial cells that attached to a collagen substrate and incorporated [3H]thymidine. Evidence for the endothelial nature of these cells was provided by the presence of Factor VIII antigen and angiotensin converting enzyme activity and by the failure of platelets to adhere to the cell surface. In addition, the cells were joined together by tight junctions. Thus, primary cultures of these cells retained both endothelial and blood-brain barrier features.