Heterogeneity of tumor microenvironment is associated with clinical prognosis of non-clear cell renal cell carcinoma: a single-cell genomics study.
Heterogeneity of tumor microenvironment is associated with clinical prognosis of non-clear cell renal cell carcinoma: a single-cell genomics study.
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肿瘤微环境异质性与非透明细胞肾细胞癌临床预后相关：一项单细胞基因组学研究
DOI：
10.1038/s41419-022-04501-9
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发表时间：
2022-01-11
影响因子：
9
通讯作者：
Cui XG
中科院分区：
文献类型：
--
作者：
Chen WJ;Cao H;Cao JW;Zuo L;Qu FJ;Xu D;Zhang H;Gong HY;Chen JX;Ye JQ;Gan SS;Zhou W;Zhu DW;Pan XW;Cui XG
Non-clear renal cell carcinomas (nccRCCs) are less frequent in kidney cancer with histopathological heterogeneity. A better understanding of the tumor biology of nccRCC can provide more effective treatment paradigms for different subtypes. To reveal the heterogeneity of tumor microenvironment (TME) in nccRCC, we performed 10x sing-cell genomics on tumor and normal tissues from patients with papillary renal cell carcinoma (pRCC), chromophobe RCC (chrRCC), collecting duct carcinoma (CDRCC) and sarcomatoid RCC (sarRCC). 15 tissue samples were finally included. 34561 cells were identified as 16 major cell clusters with 34 cell subtypes. Our study presented the sing-cell landscape for four types of nccRCC, and demonstrated that CD8+ T cells exhaustion, tumor-associated macrophages (TAMs) and sarcomatoid process were the pivotal factors in immunosuppression of nccRCC tissues and were closely correlated with poor prognosis. Abnormal metabolic patterns were present in both cancer cells and tumor-infiltrating stromal cells, such as fibroblasts and endothelial cells. Combined with CIBERSORTx tool, the expression data of bulk RNA-seq from TCGA were labeled with cell types of our sing-cell data. Calculation of the relative abundance of cell types revealed that greater proportion of exhausted CD8+ T cells, TAMs and sarRCC derived cells were correlated with poor prognosis in the cohort of 274 nccRCC patients. To the best of our knowledge, this is the first study that provides a more comprehensive sight about the heterogeneity and tumor biology of nccRCC, which may potentially facilitate the development of more effective therapies for nccRCC.
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影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
影响因子：
3.8
作者：
Drev D;Bileck A;Erdem ZN;Mohr T;Timelthaler G;Beer A;Gerner C;Marian B
通讯作者：
Marian B
影响因子：
16.6
作者：
Lake, Blue B.;Chen, Song;Jain, Sanjay
通讯作者：
Jain, Sanjay
影响因子：
6.1
作者：
Gagliardi, Filippo;Narayanan, Ashwin;Galli, Rossella
通讯作者：
Galli, Rossella
影响因子：
48
作者：
Aibar, Sara;Gonzalez-Blas, Carmen Bravo;Aerts, Stein
通讯作者：
Aerts, Stein