Extension and shrinkage of polymer brush grafted onto porous membrane induced by protein binding
Extension and shrinkage of polymer brush grafted onto porous membrane induced by protein binding
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DOI：
10.1021/ma9819642
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发表时间：
2000-02-22
期刊：
影响因子：
5.5
通讯作者：
Sugo, T
中科院分区：
文献类型：
--
作者：
Kawai, T;Sugita, K;Sugo, T
通过将聚甲基丙烯酸缩水甘油酯的环氧基团接枝到st多孔中空纤维膜上，分别与二乙胺和亚硫酸钠反应，在聚合物刷上引入了二乙氨基和磺酸基团。氨基酰化酶溶液在恒定的渗透压力下渗透通过孔，并且连续监测流出物的蛋白质浓度和渗透压力的变化。氨基酰化酶结合在多层的聚合物刷基于离子交换相互作用。含二乙氨基的聚合物刷随着蛋白质的捕获而延伸，而含磺酸基团的聚合物刷随着蛋白质结合的进展而收缩。
Two kinds of ionizable groups, namely, diethylamino and sulfonic acid groups, were introduced to a polymer brush by reaction of the epoxy groups of poly(glycidyl methacrylate) grafted onto st porous hollow-fiber membrane with diethylamine and sodium sulfite, respectively. Aminoacylase solution was permeated through the pores under a constant permeation pressure, and the changes in both the protein concentration of the effluent and the permeation pressure were continuously monitored. Aminoacylase was bound in multilayers to the polymer brush based on an ion-exchange interaction. The diethylamino-group-containing polymer brush extended with the capture of the protein, whereas the sulfonic acid-group-containing polymer blush shrank with the progression of protein binding.