Intravitreal injection of β-crystallin B2 improves retinal ganglion cell survival in an experimental animal model of glaucoma
Intravitreal injection of β-crystallin B2 improves retinal ganglion cell survival in an experimental animal model of glaucoma
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DOI：
10.1371/journal.pone.0175451
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发表时间：
2017-04-06
期刊：
影响因子：
3.7
通讯作者：
Prokosch, Verena
中科院分区：
文献类型：
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作者：
Anders, Fabian;Teister, Julia;Prokosch, Verena
本研究的目的是首先研究动物青光眼模型过程中视网膜内的特异性蛋白质组学变化，其次通过解决这些特异性变化来确定青光眼神经保护和治疗选择的新方法。通过烧灼外膜静脉使成年大鼠眼压升高。采用质谱法、光学相干断层扫描、免疫组织化学横切面和平载染色检测分子和形态变化。通过对1500多种视网膜蛋白进行定量分析，我们发现HspB5蛋白和许多β -结晶蛋白在不同的IOP升高时期表现出统一而独特的转移表达模式。晶体蛋白表达显著下调(p
Purpose of this study was to investigate firstly specific proteomic changes within the retina in the course of an animal glaucoma model and to identify secondly new approaches for neuroprotective, therapeutic options in glaucoma by addressing those specific changes. Intraocular pressure was elevated through cauterization of episcleral veins in adult Sprague Dawley rats. Molecular and morphological changes were surveyed using mass spectrometry, optical coherence tomography as well as immunohistochemical cross section- and flat mount stainings. By quantifying more than 1500 retinal proteins, it was found that the HspB5 protein and numerous beta-crystallins showed a uniform and unique shifting expression pattern as a result of different periods of elevated IOP exposure. Crystallins showed a significant downregulation (p