PROTEIN TRANSFER OF PREFORMED MHC-PEPTIDE COMPLEXES SENSITIZES TARGET-CELLS TO T-CELL CYTOLYSIS
PROTEIN TRANSFER OF PREFORMED MHC-PEPTIDE COMPLEXES SENSITIZES TARGET-CELLS TO T-CELL CYTOLYSIS
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DOI：
10.1016/1074-7613(94)90050-7
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发表时间：
1994-10-01
期刊：
影响因子：
32.4
通讯作者：
TYKOCINSKI, ML
中科院分区：
文献类型：
--
作者：
HUANG, JH;GETTY, RR;TYKOCINSKI, ML
Recombinant GPI-anchored HLA-A2.1 (HLA-A2.1-GPI/beta(2)m) was used as a protein transfer vehicle to deliver a hepatitis B virus antigenic peptide to the surfaces of cytotoxic T cell targets. Empty HLA-A2.1-GPI/beta(2)m was first produced in D. melanogaster cotransfectants and immunoaffinity purified. Cell coating with HLA-2.1-GPI/beta(2)m was shown to occur rapidly, and to be protein concentration dependent. Protein-transferred HLA-A2.1-GPI/beta(2)m effectively presented a hepatitis B virus peptide to peptide-specific HLA-A2.1-restricted T cell crones in cytotoxicity assays. Protein transfer of functional GPI-modified class I MHC-antigenic peptide complexes represents a never strategy for delivering functional antigenic complexes to cell surfaces that bypasses limitations of gene transfer and permits control of antigenic peptide densities at cell surfaces.
