Functional relevance of luteinizing hormone receptor in mouse uterus.
Functional relevance of luteinizing hormone receptor in mouse uterus.
复制标题
小鼠子宫中黄体生成素受体的功能相关性。
DOI：
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发表时间：
1994
影响因子：
5.8
通讯作者：
S. Bhattacharya
中科院分区：
文献类型：
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作者：
D. Mukherjee;P. Manna;S. Bhattacharya
据报道，猪、兔、大鼠和人子宫中存在高亲和力黄体生成素 (LH)/人绒毛膜促性腺激素 (hCG) 受体。我们已经证明 [125I]LH 与小鼠子宫的结合是可饱和的。 Scatchard图分析表明Kd为1.37 x 10(-10) mol/l，最大结合能力为5.24 nmol/kg蛋白质。已尝试观察小鼠子宫中促性腺激素受体的功能相关性。由于卵巢切除术，子宫的大小和重量显着减小；与 OVX 对照相比，给予卵巢切除 (OVX) 小鼠 LH 显着增加子宫重量 (p < 0.01)，表明 LH 对子宫有直接影响。与完整对照组相比，LH 处理的 OVX 小鼠的子宫抗坏血酸含量减少了两倍。 LH 导致 OVX 小鼠子宫重量增加是由于子宫蛋白质合成增加。 LH 对 OVX 小鼠子宫的刺激作用似乎是通过类固醇激素介导的，因为它显着增强了子宫线粒体类固醇生成。由于已知 17 β-雌二醇 (E2) 会刺激子宫蛋白质合成，因此在完整小鼠、OVX 和 OVX + LH 处理的小鼠中测定了 E2 的循环水平。与对照组相比，OVX 小鼠的 E2 循环水平下降，而 LH 治疗 7 天（注射 3 次）显着升高 OVX 小鼠的 E2 水平（p < 0.001）。 OVX 小鼠中较高的 E2 水平在肾上腺切除术中保持不变，表明肾上腺不是 E2 水平升高的来源。（摘要截断为 250 字）
The presence of high-affinity luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptors has been reported in porcine, rabbit, rat and human uteri. We have demonstrated binding of [125I]LH to mouse uterus, which was saturable. Scatchard plot analysis indicated Kd to be 1.37 x 10(-10) mol/l and the maximum binding capacity to be 5.24 nmol/kg protein. Attempts have been made to observe the functional relevance of gonadotropin receptor in the mouse uterus. The size and weight of the uterus remarkably decreased as a result of ovariectomy; administration of LH to ovariectomized (OVX) mice significantly increased the uterine weight in comparison to the OVX control (p < 0.01), indicating a direct effect of LH on the uterus. There was a two-fold decrease of uterine ascorbic acid content in LH-treated OVX mice as compared to the intact control. The gain in uterine weight of OVX mice by LH was due to the increase in uterine protein synthesis. The stimulatory effect of LH on OVX mice uterus appears to be mediated via steroid hormones because it significantly augmented uterine mitochondrial steroidogenesis. Since 17 beta-estradiol (E2) is known to stimulate uterine protein synthesis, the circulatory level of E2 was determined in intact, OVX and OVX + LH-treated mice. A fall in the circulatory level of E2 occurred in OVX mice as compared to the control, while treatment of LH for 7 days (three injections) significantly elevated E2 levels in OVX mice (p < 0.001). This higher level of E2 in OVX mice remains unaltered on adrenalectomy, indicating that adrenals are not the source for increased E2 levels.(ABSTRACT TRUNCATED AT 250 WORDS)