Identification of Peroxisome-Dividing Ring in Cyanidioschyzon merolae Based on Organelle Partner Hypothesis
Identification of Peroxisome-Dividing Ring in Cyanidioschyzon merolae Based on Organelle Partner Hypothesis
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DOI：
10.1508/cytologia.77.515
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发表时间：
2012-12-01
期刊：
影响因子：
1
通讯作者：
Kuroiwa, Tsuneyoshi
中科院分区：
文献类型：
--
作者：
Imoto, Yuuta;Kuroiwa, Haruko;Kuroiwa, Tsuneyoshi
真核细胞至少含有3种双膜结合细胞器(细胞核、线粒体和叶绿体)和4种单膜结合细胞器(内质网、高尔基体、溶酶体(液泡)和过氧化物体(微体))。这些膜结合细胞器不是从头形成的，而是在细胞周期中通过生长、分裂和分离而继承的。双膜结合细胞器是单个膜结合细胞器遗传(分裂和分离)的主导器官。胞核分裂伴随着内质网和高尔基体，而线粒体分裂伴随着过氧化物体和溶酶体。叶绿体独立分裂。尤其是，尽管过氧化物体作为线粒体分裂的亲密伙伴进行分裂，但通过传统的电子显微镜固定，尚未在分裂部位观察到过氧化物酶体分割环。因此，我们利用化学固定和高压冷冻固定的方法检测了原始红藻Merolae蓝藻细胞中可能存在的过氧化物体分裂环。通过高压冷冻固定，我们能够在分裂部位识别出一个电子致密的过氧化物体分割环。
Eukaryotic cells contain at least 3 types of double membrane-bound organelles (cell nucleus, mitochondrion and chloroplast (plastid), and 4 types of single membrane-bound organelles (endoplasmic reticulum (ER), Golgi apparatus, lysosome (vacuole) and peroxisome (microbody)). These membrane-bounded organelles cannot be formed de novo but are inherited by growth, division and separation during cell cycle. The double membrane-bounded organelle plays a role as a leading apparatus of the inheritance (division and separation) of the single membrane-bounded organelles. The cell-nucleus divides accompanied by ER and Golgi apparatus while the mitochondra divide accompanied by a peroxisome and lysosomes. Chloroplasts divide independently. In particular, although the peroxisome divides as an intimate partner of mitochondrial division, the peroxisome-dividing ring has not yet been observed at the division site by traditional electron microscopic fixation. So we examined the putative peroxisome-dividing ring in the primitive red alga Cyanidioschyzon merolae cells by use of chemical fixation and high pressure freeze fixation. We were able to identify an electron dense peroxisome-dividing ring at the division site by use of high pressure freeze fixation.